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赵文溪,刘莹,于超勇,王丽娟,宋爱环,刘洪军,张树东,官曙光.基于CR及Cytb基因序列的大泷六线鱼野生群体遗传多样性分析[J].广西科学院学报,2019,35(4):308-318. [点击复制]
- ZHAO Wenxi,LIU Ying,YU Chaoyong,WANG Lijuan,SONG Aihuan,LIU Hongjun,ZHANG Shudong,GUAN Shuguang.Analysis of Genetic Diversity of Wild Populations of Hexagrammos otakiibased on CRand CytbGene Sequences[J].Journal of Guangxi Academy of Sciences,2019,35(4):308-318. [点击复制]
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| 基于CR及Cytb基因序列的大泷六线鱼野生群体遗传多样性分析 |
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赵文溪1, 刘莹1, 于超勇1, 王丽娟2, 宋爱环1, 刘洪军1, 张树东3, 官曙光1
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| (1.山东省海洋生物研究院, 青岛市浅海底栖渔业增殖重点实验室, 青岛市海洋生物种质资源挖掘与利用工程实验室, 山东青岛 266104;2.中国科学院海洋研究所, 中国科学院实验海洋生物学重点实验室, 山东青岛 266071;3.日照市水产集团总公司, 山东日照 276800) |
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| 摘要: |
| 为进一步了解山东省内大泷六线鱼（Hexagrammos otakii）群体遗传背景和分化情况，合理保护与开发利用渔业资源，选取线粒体控制区（CR）和细胞色素b基因（Cytb）对分布于山东省近海沿岸的3个大泷六线鱼野生群体以及北黄海1个离岸大泷六线鱼野生群体的线粒体基因序列进行对比，分析它们的序列特征、遗传多样性和种群历史动态情况。经PCR扩增得到大泷六线鱼野生群体的CR和Cytb基因序列，全长分别为485 bp和651 bp。基于CR基因序列共检测到40个多态位点，单倍型多样性平均值为0.908，核苷酸多样性平均值为0.006，定义了53种单倍型。基于Cytb基因序列检测到56个多态位点，转换颠换比值为19.04，种内单倍型多样性平均值为0.934，核苷酸多样性平均值为0.005，定义了38种单倍型。对比分析表明，4个大泷六线鱼野生群体均具有高单倍型多样性和低核苷酸多样性。NJ系统进化树、群体间/内平均遗传距离、Fst值、基因流及AMOVA分析结果显示：山东省内近海沿岸大泷六线鱼野生群体和北黄海离岸野生群体遗传差异不显著，群体间存在频繁的基因交流，未形成显著的遗传分化。种群动态结果表明大泷六线鱼于更新世晚期经历了快速扩张，并形成了现有遗传格局。山东省内近海沿岸大泷六线鱼野生群体和北黄海离岸野生群体遗传结构之间不存在显著的地理隔离，这可能与人为增殖放流和秋冬季节黄海沿岸流及暖流有关，从而使得群体之间基因交流广泛。 |
| 关键词: 大泷六线鱼 线粒体DNA 遗传多样性 线粒体基因控制区 细胞色素b基因 群体遗传结构 |
| DOI：10.13657/j.cnki.gxkxyxb.20191129.001 |
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| 基金项目:山东省现代农业产业体系鱼类产业创新团队项目（SDAIT-12-02），市南区科技发展资金项目（2018-4-004-ZH），山东省自然科学基金-青年基金项目（ZR2016DQ03），山东省农业重大应用技术创新项目"生态工程化尾水处理系统的构建与应用技术研究"和烟台市科技计划项目（2018SFBF084，2019YT06340852）资助。 |
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| Analysis of Genetic Diversity of Wild Populations of Hexagrammos otakiibased on CRand CytbGene Sequences |
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ZHAO Wenxi1, LIU Ying1, YU Chaoyong1, WANG Lijuan2, SONG Aihuan1, LIU Hongjun1, ZHANG Shudong3, GUAN Shuguang1
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| (1.Qingdao Key Laboratory of Berthic Fisheries Aquaculture and Enhancement, Qingdao Engineering Laboratory of Exploration and Utilization of Marine Germ Plasm Resources of Qingdao, Marine Biology Institute of Shandong Province, Qingdao, Shandong, 266104, China;2.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, 266071, China;3.Rizhao Fisheries Group General Corparation, Rizhao, Shandong, 276800, China) |
| Abstract: |
| In order to further understand the genetic background and differentiation of Hexagrammos otakii population in Shandong Province and rationally protect and exploit fishery resources, the mitochondrial control region (CR) and cytochrome b gene (Cytb) were selected to compare mitochondrial gene sequences of three wild populations of Hexagrammos otakii distributed along the coast of Shandong Province and one wild population of North Yellow Sea offshore, and their sequence characteristics, genetic diversity and historical dynamics of population were analyzed. The CR and Cytb gene sequences of Hexagrammos otakii wild populations were obtained by PCR amplification, with the total length of 485 bp and 651 bp, respectively. A total of 40 polymorphic sites were detected based on CR gene sequence, with an average haplotype diversity of 0.908 and an average nucleotide diversity of 0.006.53 haplotypes were defined. Based on Cytb gene sequence, 56 polymorphic sites were detected. The transition/transversion ratio was 19.04, the average value of intra-specific haplotype diversity was 0.934, and the average value of nucleotide diversity was 0.005, thus 38 hapotypes were defined. Comparative analysis showed that all wild population sequences of four wild Hexagrammos otakii had high haplotype diversity and low nucleotide diversity. NJ phylogenetic tree, average genetic distance between populations/within populations, Fst value, gene flow and AMOVA analysis results showed that there was no significant genetic difference between wild populations of Hexagrammos otakii along the coast of Shandong Province and North Yellow Sea offshore wild populations, there was frequent gene exchange among populations, and no significant genetic differentiation was formed. The results of population dynamics showed that Hexagrammos otakii underwent rapid expansion in the late Pleistocene and formed the existing genetic pattern. There is no significant geographical isolation between the genetic structure of the wild populations of Hexagrammos otakii along the coast of Shandong Province and the wild population of North Yellow Sea offshore, which may be related to human proliferation and release and the Yellow Sea coastal currents and warm currents in autumn and winter seasons, which may lead to extensive gene communication between populations. |
| Key words: Hexagrammos otakii mitochondrial DNA genetic diversity mitochondria control region cytochrome b gene population genetic structure |
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