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青岛中港微型生物膜中污损细菌群落初探
马士德1,2, 张林林3, 段继周1,4, 王在东5, 刘瑞1,4, 马岩1,4, 韩文5, 孙超岷1,4
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(1.中国科学院海洋研究所, 山东青岛 266071;2.中国工业防腐蚀技术协会海洋防腐防污专业委员会, 山东青岛 266071;3.山东科技大学化学与环境工程学院, 山东青岛 266590;4.青岛海洋科学与技术国家实验室, 山东青岛 266237;5.青岛东启机械设备有限公司, 山东青岛 266071)
摘要:
本研究拟通过揭示微型生物膜中的细菌群落特征,为污损生物学和海洋腐蚀学的微观研究提供参考和启示。试验采用3种方法:传统稀释涂布法、流式细胞仪计数法以及16s rDNA扩增子高通量测序对青岛中港冬季海水以及玻璃板中微型生物膜中的污损细菌群落结构进行分析。试验结果表明冬季海水表层和底层中细菌的浓度分别为3.0×108 CFU/L和6.9×107 CFU/L,表层和底层玻璃板微型生物膜中的细菌浓度分别为7.2×107 CFU/L和2.1×107 CFU/L,海水和玻璃板中的细菌以Proteobacteria和Bacteroidetes为主。另外,海水中细菌群落结构组成与玻璃板微型生物膜中细菌具有较高的一致性。本研究利用不同的试验方法揭示微型生物膜中的细菌群落特征,对深入研究生物污损,从根本上解决海洋生物污损问题、制定绿色防污措施具有重要的现实意义。
关键词:  微型生物膜  污损细菌群落  结构分析
DOI:10.13657/j.cnki.gxkxyxb.20190515.006
基金项目:国家自然科学基金项目(59471054,59071040)资助。
A Preliminary Study on Bacterial Community of Micro-biofilm in Qingdao Zhonggang
MA Shide1,2, ZHANG Linlin3, DUAN Jizhou1,4, WANG Zaidong5, LIU Rui1,4, MA Yan1,4, HAN Wen5, SUN Chaomin1,4
(1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, 266071, China;2.Marine Anticorrosion and Antifouling Society's Division of CIATA, Qingdao, Shandong, 266071, China;3.College of Chemistry & Environmental Engineering, Shandong University of Science & Technology, Qingdao, Shandong, 266590, China;4.Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong, 266237, China;5.Qingdao Tony Machinery and Equipment Co., Ltd, Qingdao, Shandong, 266071, China)
Abstract:
This study aims to provide a reference and inspiration for the microscopic study of fouling biology and marine corrosion by revealing the characteristics of the bacterial community in the micro-biofilm. Three different methods, including traditional dilution coating method, flow cytometry counting method and 16 s rDNA amplifiers high-throughput sequencing, were used to analyze the fouling bacteria community structure in the micro-biofilm of Qingdao Zhonggang winter seawater and glass plate. Experimental results showed that the concentrations of bacteria in the surface layer and bottom layer of seawater in winter were 0.300×109 CFU/L and 0.069×109 CFU/L, respectively. The concentrations of bacteria in the micro-biofilm of the surface and bottom glass plates were 0.072×109 CFU/L and 0.021×109 CFU/L, respectively. The bacteria in seawater and glass plates were dominated by Proteobacteria and Bacteroidetes.In addition, the composition of the bacteria community in seawater was highly consistent with the bacteria in the micro-biofilm of the glass plate. In this paper, different experimental methods are used to reveal the characteristics of the bacteria community in the micro-biofilm, which is of great practical significance for the in-depth study of biological fouling, fundamental solution to the problem of marine biological fouling, and the formulation of green anti-fouling measures.
Key words:  micro-biofilm  fouling bacteria community  structure analysis

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