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孙玉林,叶金敏,张银,梁木清,林晓銮,董永清,陈道海.3种脱蛋白方法对虎斑乌贼肌肉多糖体外抗氧化活性的影响[J].广西科学院学报,2017,33(3):171-177. [点击复制]
- SUN Yulin,YE Jinmin,ZHANG Yin,LIANG Muqing,LIN Xiaoluan,DONG Yongqing,CHEN Daohai.Effects of Three Different Deproteinized Methods on Antioxidant Activity of Polysaccharide Extract from the Muscle of Sepiapharaonis in vitro[J].Journal of Guangxi Academy of Sciences,2017,33(3):171-177. [点击复制]
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| 3种脱蛋白方法对虎斑乌贼肌肉多糖体外抗氧化活性的影响 |
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孙玉林1,2, 叶金敏1, 张银1, 梁木清1, 林晓銮1, 董永清1, 陈道海1,2
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| (1.岭南师范学院生命科学与技术学院, 广东湛江 524048;2.环北部湾海洋药用动物利用与保护研究所, 广东湛江 524048) |
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| 摘要: |
| [目的]比较3种不同脱蛋白方法对虎斑乌贼肌肉多糖体外抗氧化活性的影响。[方法]采用Sevag法、三氯乙酸法和等电点法对经过热水浸提的虎斑乌贼肌肉多糖进行脱蛋白,同时对脱蛋白多糖的体外抗氧化活性进行研究。[结果]3种不同脱蛋白方法得到的多糖均具有一定的抗氧化能力。当多糖浓度为5 mg/mL时,Sevag法、三氯乙酸法和等电点法得到的脱蛋白多糖对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除率分别为87.05%、42.35%和38.54%,相应的IC50值分别为1.96 mg/mL、11.81 mg/mL和9.84 mg/mL;对羟基自由基(·OH)清除率分别为93.67%、70.80%和68.75%,相应的IC50值分别为0.67 mg/mL、1.37 mg/mL和2.72 mg/mL;对超氧阴离子(O2-)清除率分别为72.33%、62.42%和53.33%,相应的IC50值分别为0.15 mg/mL、1.56 mg/mL和3.81 mg/mL。多糖还原力大小分别为0.183,0.160和0.144。[结论]3种脱蛋白方法所得虎斑乌贼肌肉多糖抗氧化活性大小依次为Sevag法 > 三氯乙酸法 > 等电点法。 |
| 关键词: 虎斑乌贼 肌肉 脱蛋白 多糖 抗氧化活性 |
| DOI：10.13657/j.cnki.gxkxyxb.20170814.001 |
| 投稿时间：2017-01-10 |
| 基金项目:广东省自然科学基金项目(2015A030310406),广东省教育厅特色创新项目(2016KTSCX081),广东省省部产学研合作专项(2013B090500036)和湛江市财政资金科技专项竞争性分配项目(2013A05004,2015A06008)资助。 |
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| Effects of Three Different Deproteinized Methods on Antioxidant Activity of Polysaccharide Extract from the Muscle of Sepiapharaonis in vitro |
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SUN Yulin1,2, YE Jinmin1, ZHANG Yin1, LIANG Muqing1, LIN Xiaoluan1, DONG Yongqing1, CHEN Daohai1,2
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| (1.Life Science and Technology School, Lingnan Normal University, Zhanjiang, Guangdong, 524048, China;2.Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine, Zhanjiang, Guangdong, 524048, China) |
| Abstract: |
| [Objective] To compare the effects of three methods of deproteinization of polysaccharide from the muscle of Sepiapharaonis and its antioxidant activity in vitro.[Methods] The Sevag method, trichloroacetic acid method and isoelectric point method were adopted to strip of protein from Sepiapharaonis muscle polysaccharides. At the same time, the ability of antioxidant activity of the deproteinated polysaccharides were studied.[Results] The polysaccharides of stripping of protein by three different ways possessed good antioxidant activity. When the concentration of polysaccharides was 5 mg/mL, for the deproteinated polysaccharides obtained by the Sevag method, trichloroacetic acid method and isoelectric point method, the scavenging rate of DPPH was 87.05%,42.35% and 38.54%,respectively and the corresponding IC50 values were 1.96 mg/mL,11.81 mg/mL and 9.84 mg/mL,respectively. The scavenging ratio of ·OH was 93.67%,70.80% and 68.75%, respectively and the corresponding IC50 values were 0.67 mg/mL,1.37 mg/mL and 2.72 mg/mL, respectively. The scavenging rate of O2- free radical was 72.33%,62.42% and 53.33%,respectively and the corresponding IC50 values were 0.15 mg/mL,1.56 mg/mL and 3.81 mg/mL,respectively.The value of reducing capacity was 0.183, 0.160 and 0.144,respectively.[Conclusion] The antioxidant activities for the deproteinated polysaccharides from Sepiapharaonis muscle by three different methods were Sevag method > trichloroacetic acid method > isoelectric point method. |
| Key words: Sepiapharaonis muscle deproteinzation polysaccharides antioxidant activity |
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