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丁刚,辛美丽,李慧,吴海一,李大鹏.氨态氮浓度及藻体代谢物对裙带菜雄配子体生长的影响[J].广西科学院学报,2017,33(3):158-163. [点击复制]
- DING Gang,XIN Meili,LI Hui,WU Haiyi,LI Dapeng.Ammonia Nitrogen Concentration and the Accumulation of Algal Metabolites on the Growth of Male Gametophyte of Undaria pinnatifida[J].Journal of Guangxi Academy of Sciences,2017,33(3):158-163. [点击复制]
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| 氨态氮浓度及藻体代谢物对裙带菜雄配子体生长的影响 |
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丁刚1,2, 辛美丽1,2, 李慧3, 吴海一1,2, 李大鹏1,2
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| (1.山东省海洋生物研究院, 山东青岛 266104;2.青岛市大型海藻工程技术研究中心, 山东青岛 266104;3.温州大学生命与环境科学学院, 浙江温州 325035) |
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| 摘要: |
| [目的]探讨氨态氮浓度对裙带菜雄配子体生长的影响,找出适合在实验室中培养裙带菜雄配子体的氮浓度。[方法]将裙带菜雄配子体置于不同氮浓度的氮培养液和新旧培养液中培养,用PANLUX照度计测量穿透光强,并计算出消光值;用次溴酸盐氧化法测定溶液中氨氮浓度;记录藻体的初始重量及实验结束时的重量,由此计算特定生长率。[结果]在氨态氮浓度为15 mg/L左右时,裙带菜雄配子体的平均日生长率约为17.7%,显示出良好的长势。相比之下,过高的氨氮浓度不利于藻体的生长。[结论]在一定范围内,提高氮浓度会加快配子体克隆的生长,但过高的氮浓度会影响它的发育。培养液中积累的藻体代谢物对藻体生长没有明显的抑制作用。 |
| 关键词: 裙带菜 雄配子体 氮浓度 |
| DOI：10.13657/j.cnki.gxkxyxb.20170516.001 |
| 投稿时间：2017-04-10 |
| 基金项目:山东省现代农业技术体系建设专项资金项目(SDAIT-26号)和山东省农业重大应用技术创新项目资助。 |
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| Ammonia Nitrogen Concentration and the Accumulation of Algal Metabolites on the Growth of Male Gametophyte of Undaria pinnatifida |
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DING Gang1,2, XIN Meili1,2, LI Hui3, WU Haiyi1,2, LI Dapeng1,2
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| (1.Marine Biology Institute of Shandong Province, Qingdao, Shandong, 266104, China;2.Ma-croalgae Engineering Technology Research Centre of Qingdao, Qingdao, Shandong, 266104, China;3.College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang, 325035, China) |
| Abstract: |
| [Objective] To investigate the effect of ammonia concentration on the male gametophyte growth of Undaria pinnatifida, and to find out the nitrogen concentration suitable for culturing the gametophyte.[Methods] The male gametophyte of Undaria pinnatifida was cultivated in different nitrogen concentrations and cultured in old and new medium.The penetration intensity was measured with the PANLUX and the extinction value was calculated.The concentration of ammonia nitrogen in the solution was determined by the dibromate oxidation.The initial and final weights of the algae were recorded.So the specific growth rate could be calculated.[Results] When the concentration of ammonia nitrogen was about 15 mg/L,the average daily growth rate of the male gametophyte was about 17.7%,showing good growth.In contrast,the high ammonia nutrition concentration was not conducive to the growth of algae.[Conclusion] In a certain range,increasing the nitrogen concentration will accelerate the growth of gametophyte clones,but too high nitrogen concentration will affect its development.The algae metabolites accumulated in the culture medium did not significantly inhibit the growth of algae. |
| Key words: Undaria pinnatifida male gametophyte nitrogen concentration |
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