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海芒果可培养细菌的分离鉴定及其抑制海洋鱼类致病菌活性研究
李小群1,2, 李菲1, 李家怡1,2, 颜栋美2, 苏志维1, 高程海1,3
0
(1.广西科学院, 广西海洋天然产物与组合生物合成化学重点实验室培育基地, 广西南宁 530007;2.广西大学轻工与食品工程学院, 广西南宁 530004;3.广西海洋研究所广西海洋生物技术重点实验室, 广西北海 536000)
摘要:
[目的]研究红树植物海芒果Cerbera manghas L.及其根际土壤可培养细菌多样性及其代谢产物抑制鱼类致病菌的生物活性。[方法]采用纯培养法分离并基于16S rRNA基因序列的系统发育分析法对分离得到的细菌进行多样性研究,采用滤纸片法研究其抑制海洋鱼类致病菌的生物活性。[结果]从海芒果及其根际土壤中共分离得到15株可培养细菌,采用16S rRNA基因序列的系统发育分析后发现,这些菌分属于2个大的细菌发育类群:厚壁菌门(Firmicutes,占比为53%)和放线菌门(Actinobacteria,占比为47%),其中4株为芽孢杆菌属,6株为链霉菌属。活性研究发现,所有测试菌株对3种海洋鱼类致病弧菌均有一定的抑制作用,其中VT86(Kitasatospora paranensis),V139-2(Streptomyces luteireticuli),V140-2(Streptomyces jiujiangensis),VT19(Streptomyces orinoci),VT113(Streptomyces niveiscabiei)这5株菌的抑菌效果较好,以VT86(K.paranensis)抑菌活性最好。[结论]红树植物海芒果及其根际土壤中的细菌丰富多样,部分细菌具有抑制海洋鱼类致病菌生长的生物活性,具有开发成新型抑制鱼类致病菌药物的潜力。
关键词:  海芒果  可培养细菌  系统发育分析  抑菌活性
DOI:10.13657/j.cnki.gxkxyxb.20170509.001
投稿时间:2016-04-18
基金项目:国家自然科学基金项目(41566004),广西自然科学基金项目(2015GXNSFBB139001)和广西海洋生物技术重点实验室开放基金资助课题(GLMBT-201603)资助。
Identification and Inhibition Activity Inhibit Fish Pathogens of Cultivated Bacteria Isolated from Cerbera manghas
LI Xiaoqun1,2, LI Fei1, LI Jiayi1,2, YAN Dongmei2, SU Zhiwei1, GAO Chenghai1,3
(1.Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynethesis Chemistry, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi, 530004, China;3.Guangxi Key Laboratory of Marine Biotechnology, Guangxi Institute of Oceanology, Beihai, Guangxi, 536000, China)
Abstract:
[Objective] To study the bioactivity of Cerbera manghas and its rhizosphere soil to cultivate bacterial diversity and its metabolites to inhibit fish pathogens. [Methods] The diversity of isolated bacteria was studied by the method of pure culture and the phylogenetic analysis based on 16S rRNA gene sequence using the filter paper method,the isolated bacteria were tested to inhibit the activity of marine fish pathogenic bacteria.[Results] We isolated 15 bacterial strains from Cerbera manghas and its rhizosphere soil through conventional culture dependent method,identified the strains according to morphological observation,physiological,biochemical characteristics,and the homologous analysis of 16S rRNA sequences of nucleotides. Results showed that these strains belonged to two major bacterial development groups:Firmicutes (53%) and Actinobacteria (47%),of which 4 were Bacillus sp.,6 were Streptomyces.Activity showed that the VT86 had the best antibacterial activity,which might have potential value for developing new inhibition of sea fish pathogens drugs.[Conclusion] Cerbera manghas and its rhizosphere soil were rich in bacteria resources,and biological activity of those strains could improve the prevention and treatment of sea fish diseases.
Key words:  Cerbera manghas  cultured bacteria  phylogeny  antimicrobial activity

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