| 摘要: |
| [目的]完善三七花颗粒制剂的质量控制标准。[方法]采用薄层色谱法对三七花颗粒进行定性鉴别,并用高效液相色谱法对颗粒中的人参皂苷Rb3进行定量测定。[结果]三七花颗粒供试品与对照品及药材在薄层色谱中相应的位置上,显现相同颜色的荧光斑点;人参皂苷Rb3在0.505~5.050 μg成良好线性关系(r=0.999 9),平均回收率为101.25%,RSD为0.54%(n=6)。[结论]该方法简便、准确,可用于三七花颗粒质量的控制。 |
| 关键词: 高效液相色谱 薄层色谱 三七花颗粒 人参皂苷Rb3 |
| DOI：10.13657/j.cnki.gxkxyxb.20161128.001 |
| 投稿时间：2016-06-10 |
| 基金项目:广西中药质量标准研究重点实验室系统性研究课题(桂中重系201310)和广西科学研究与技术开发项目(重大专项计划,桂科重14124002-11)资助。 |
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| Study on the Quality Control Method of Sanchi GranuLe |
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FENG Jun, LIU Buming
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| (Guangxi Key Laboratory of Tradtitonal Chinese Medicine Quality Standards, Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Nanning, Guangxi, 530022, China) |
| Abstract: |
| [Objective] To perfect the quality control standard of sanchi granuLe.[Methods] The TLC method was used to identify the sanchi granuLe and the HPLC method was used for the determination of ginsenoside Rb3 in it.[Results] Each of the sanchi,sanchi granuLe and ginsenoside Rb3 had a similar fluorescent spot which performed the same colour and Rf in the TLC. The calibration curve of ginsenoside Rb3 showed a good linearity within 0.505~5.050 μg, r=0.999 9. The average recovery was 101.25%,RSD was 0.54% (n=6).[Conclusion] The method was simple and accurate,it could be used for the quality control of Sanchi GranuLe. |
| Key words: HPLC TLC sanchi granuLe ginsenoside Rb3 |
