| 引用本文： |
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罗先群,黄雪星.杏鲍菇菌株的rDNA ITS序列鉴定和同工酶分析[J].广西科学院学报,2016,32(2):156-160. [点击复制]
- LUO Xianqun,HUANG Xuexing.Analysis on Isozyme and rDNA ITS Sequence of Pleurotus eryngii Strains[J].Journal of Guangxi Academy of Sciences,2016,32(2):156-160. [点击复制]
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| 摘要: |
| [目的]鉴于诱变处理后杏鲍菇菌株在栽培性状上的明显差异,对两株杏鲍菇菌株(杏A和杏B)进行分子水平上的鉴定,分析两者的差异性。[方法]对杏鲍菇菌丝体样本的rDNA基因内转录间ITS片段进行PCR扩增并测序验证;同时应用同工酶电泳对菌丝体的酯酶、过氧化物酶和超氧化物歧化酶进行分析。[结果]通过GenBank数据库搜索和序列比对分析,两株菌株ITS序列相似度达到99%;菌丝体的酯酶、过氧化物酶和超氧化物歧化酶同工酶电泳检测无差异。[结论]杏A和杏B菌株为同一菌株,在分子水平上差异不明显。 |
| 关键词: 杏鲍菇 同工酶 rDNA ITS 序列分析 |
| DOI： |
| 投稿时间：2016-03-10 |
| 基金项目:广西科学院基本科研业务费项目(15YJ22SW20)资助。 |
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| Analysis on Isozyme and rDNA ITS Sequence of Pleurotus eryngii Strains |
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LUO Xianqun, HUANG Xuexing
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| (Biology Institute, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| [Objective] In view of the fact that the mutation of Pleurotus eryngii strains is different in the cultivated character.Two strains of Pleurotus eryngii strains (A and B) areidentified at molecular level and analyze their difference.[Methods] The rDNA fragment of Pleurotus eryngii mycelium samples was amplified by PCR and sequencing.Mycelium of esterase,peroxidase and superoxide dismutase enzyme are analyzed by isozyme electrophoresis.[Results] Through the analysis of GenBank database search and sequence alignment, the similarity of ITS sequences of the two strains was reached to 99%; There was no difference between esterase, peroxidase and superoxide dismutase isozymes in the mycelium.[Conclusion] A and B strain is the same strain, at the molecular level with insignificant difference. |
| Key words: Pleurotus eryngii isoenzyme rDNA ITS sequence analysis |
