| 引用本文： |
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苏志维,马仲辉,梁益其,何碧娟,陈波.红树植物卤蕨地上部分提取物的生物活性初报[J].广西科学院学报,2015,31(4):292-295. [点击复制]
- SU Zhi-wei,MA Zhong-hui,LIANG Yi-qi,HE Bi-juan,CHEN Bo.Study on Biological Activity of Extracts from the Aerial Part of Mangrove Plant Acrostichum aureum[J].Journal of Guangxi Academy of Sciences,2015,31(4):292-295. [点击复制]
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| 摘要: |
| [目的]研究红树植物卤蕨(Acrostichum aureum)地上部分的乙醇提取物及其不同极性部位萃取物对我国3种高发性肿瘤细胞株(MCF-7,A549,HepG2)和3种微生物菌株(金黄色葡萄球菌Staphyloccocus aureus Rosenbach、大肠杆菌Escherichia coli、痤疮丙酸杆菌Propionibacterium acnes)的生长抑制作用。[方法]采用MTT法测定其对所选3种人肿瘤细胞株的细胞毒活性;采用改良的刃天青显色法,测定对3种微生物菌株的体外抗菌作用的最低抑菌浓度(MIC)。[结果]卤蕨地上部分的不同极性部位提取物对所选人肿瘤细胞株和微生物菌株表现出不同程度的生长抑制作用,其中乙酸乙酯萃取部位对3种细胞株具有很好的细胞毒活性,IC50值均低于20 μg/mL,对金黄色葡萄球菌、大肠杆菌和痤疮丙酸杆菌的MIC分别为125 μg/mL、125 μg/mL、62.5 μg/mL;正丁醇萃取部位的细胞毒活性和抑菌作用次之;石油醚萃取部位和水层对所选细胞株和菌株几乎不表现抑制作用。[结论]卤蕨地上部分乙醇提取物的乙酸乙酯萃取部位具有较好的细胞毒活性和抑菌作用,值得进行进一步活性追踪、分离纯化,以期获得具有药用价值的活性先导化合物。 |
| 关键词: 卤蕨 提取物 细胞毒活性 抑菌活性 |
| DOI： |
| 投稿时间：2015-05-13修订日期：2015-05-19 |
| 基金项目:广西科学院基本科研业务费项目(15YJ22HY06),广西自然科学青年基金项目(2015GXNSFBA139026),广西自然科学基金项目(2012GXNSFEA053001)和广西中药质量标准研究重点实验室开放课题项目(桂中重开201408)资助。 |
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| Study on Biological Activity of Extracts from the Aerial Part of Mangrove Plant Acrostichum aureum |
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SU Zhi-wei1, MA Zhong-hui2, LIANG Yi-qi3, HE Bi-juan1, CHEN Bo1
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| (1.Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.College of Agriculture, Guangxi University, Nanning, Guangxi, 530003, China;3.South China Botanical Garden, CAS, Guangzhou, Guangdong, 510650, China) |
| Abstract: |
| [Objective] The cytotoxic activities and the minimal inhibitory concentration (MIC) of various extractions of the aerial part of Acrostichum aureum were investigated respectively on three human cancer cell lines (MCF-7,A549,HepG2) and microorganisms(Staphyloccocus aureus Rosenbach,Escherichia coli,Propionibacterium acnes).[Methods] The cytotoxic activities were tested in vitro by MTT assay.The MIC values were determined by improved resazurin colorimetric method.[Results] The various extractions of the aerial part of A.aureum display different levels of cytotoxic and antimicrobial activities.The ethyl acetate extract was exhibited noticeable cytotoxicity against the tested cancer cell lines (IC50<20 μg/mL),as well as show potent antimicrobial activities with the MIC values of 125 μg/mL,125 μg/mL and 62.5 μg/mL against Staphyloccocus aureus Rosenbach,Escherichia coli and Propionibacterium acnes,respectively.The cytotoxic and antimicrobial activities of n-butanol extract was next to that of ethyl acetate extract.The petroleum ether and water soluble part were found to be no effects on all the tested cancer cell lines and microorganism lines.[Conclusion] The ethyl acetate extract is exhibited noticeable cytotoxicity and antimicrobial activities,which is worth to further investigate the constituents of A.aureum by activity tracking assay to develop of more lead compounds. |
| Key words: Acrostichum aureum extractions cytotoxicity antimicrobial activity |
