| 引用本文： |
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辛美丽,吕芳,孙福新.一株具有降解性能的巨大芽孢杆菌的鉴定与发酵条件优化[J].广西科学院学报,2015,31(4):247-252. [点击复制]
- XIN Mei-li,LV Fang,SUN Fu-xin.Identification and Optimization of Fermentation Condition for Bacillus megaterium from Sea Cucumber Ponds[J].Journal of Guangxi Academy of Sciences,2015,31(4):247-252. [点击复制]
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| 摘要: |
| [目的]对从刺参养殖池塘中分离得到的降解菌株B11进行菌种鉴定,并对其发酵条件进行优化。[方法]经生化及16S rDNA鉴定该菌种的种属;利用单因素筛选和正交试验设计对其发酵条件进行研究。[结果]经鉴定该菌株为一株巨大芽孢杆菌,最优发酵条件是装液量为70 mL/250 mL,转速为180 r/min,培养温度为28℃,pH值为8。[结论]菌株B11在最优发酵条件下生长良好,满足工业化大规模扩大培养所需条件,有较好的应用前景。 |
| 关键词: 巨大芽孢杆菌 刺参 正交实验 发酵条件 |
| DOI： |
| 投稿时间：2015-07-20 |
| 基金项目:海洋公益性行业科研专项经费项目(201305005),海洋经济创新发展区域示范项目和青岛市市南区科技发展资金项目(2013-12-005-SW)资助。 |
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| Identification and Optimization of Fermentation Condition for Bacillus megaterium from Sea Cucumber Ponds |
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XIN Mei-li1,2, LV Fang1,2, SUN Fu-xin1
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| (1.Marine Biology Institute of Shandong Province, Qingdao, Shandong, 266104, China;2.Macroalgae Engineering Technology Center of Qingdao, Qingdao, Shandong, 266104, China) |
| Abstract: |
| [Objective] A bacterium named B11 capable of organic-pollutants degradation, isolated from sea cucumber external culturing ponds, was identified and the liquid fermentation condition of B11 was optimized.[Methods] The genus of B11 was studied through physiological and bio-chemical tests and 16S rDNA sequence similarity analysis.The condition of liquid fermentation for bacterium B11 were optimized by mono-factor experiment and uniform design.[Results] B11 was preliminarily identified Bacillus megaterium.The optimized fermentation condition was at pH 8 with 70 mL production medium inoculated with 5% seed culture and incubated at 28℃,180 r/min.[Conclusion] B11 grew well under optimal fermentation conditions and it could satisfy the requirement for industrial large-scale cultivation, which shows a good prospect for industrial application in future. |
| Key words: Bacillus megaterium sea cucum-ber orthogonal experiment fermentation conditions |
