| 引用本文： |
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张红岩,周兴,莫勇生,唐峰,孙文波.兰州百合组织培养及快速繁殖技术研究[J].广西科学院学报,2015,31(1):49-53. [点击复制]
- ZHANG Hong-yan,ZHOU Xing,MO Yong-sheng,TANG Feng,SUN Wen-bo.Studies on Tissue Culture and Rapid Propagation of Lifium davidii var.Unicolor (Hoog) Cotton[J].Journal of Guangxi Academy of Sciences,2015,31(1):49-53. [点击复制]
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| 摘要: |
| [目的]建立适于兰州百合(Lifium davidii var.Unicolor(Hoog) Cotton)的快速无性繁殖技术,为食用兰州百合试管苗规模化生产提供技术参考。[方法]以球根鳞片为外植体,采用不同消毒方法、取材部位、接种方法和植物生长调节剂及其浓度配比,筛选适合的诱导、增殖和生根培养基。[结果]球根鳞片先用75%酒精处理后,用10%的次氯酸钠与0.13%的升汞等体积混合液消毒,再用0.13%升汞消毒为最佳消毒方式;接种选择与鳞茎盘相连的下部鳞片为初代培养的最佳外植体;外植体在MS+6-BA 1.5 mg/L+GA30.8 mg/L+2,4-D 1.0 mg/L的培养基中,能诱导出较多长势健壮的不定芽;在MS+6-BA 1.0 mg/L+NAA 0.1 mg/L的培养基中,增殖倍数达1.25;而生根培养以MS+NAA 0.6 mg/L+IBA 0.1 mg/L为最佳,生根苗生长良好,生根率达100%。[结论]获得适合兰州百合快速繁殖的培养基配方,建立了兰州百合的快速繁殖技术。 |
| 关键词: 兰州百合 外植体 鳞片 离体培养 |
| DOI： |
| 投稿时间：2014-10-12修订日期：2014-12-09 |
| 基金项目:广西科学院基本科研业务费资助项目(12YJ25SWS22)资助。 |
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| Studies on Tissue Culture and Rapid Propagation of Lifium davidii var.Unicolor (Hoog) Cotton |
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ZHANG Hong-yan, ZHOU Xing, MO Yong-sheng, TANG Feng, SUN Wen-bo
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| (Biology Institute, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| [Objective] The technology of rapid clonal propagation for Lifium davidii var.Unicolor (Hoog) Cotton was established. It can be used for Lifium davidii var.Unicolor (Hoog) Cotton tube seedlings factory production.[Methods] Using the bulb scales as the explants, the effects of different sterilization method, test types, inoculation methods, and plant growth regulators with different concentration on inducement and proliferation were compared.[Results] The results showed that the optimal disinfection method was 75% alcohol treatments followed with the mixture of 10% sodium hypochlorite and 0.1% corrosive sublimate (1:1) and 0.1% corrosive sublimate. The optimal explants for primary cultured was the lower scales that connect to vittata. The best induction medium was MS+6-BA 1.5 mg/L+GA3 0.8 mg/L+2,4-D 1.0 mg/L. It could induce more and stronger shoots. The optimal scales medium for subculture was MS with 6-BA 1.0 mg/L+NAA 0.1 mg/L. The optimal medium for rooting was MS+NAA 0.6 mg/L+IBA 0.1 mg/L with 100% the rooting rate.[Conclusion] The optimal rapid propagation medium for Lifium davidii var.Unicolor (Hoog) Cotton was identified. The high tissue culture and rapid propagation system of Lifium davidii var.Unicolor (Hoog) Cotton was established. |
| Key words: Lifium davidii var.Unicolor (Hoog) Cotton explant scales isolated culture |
