| 引用本文： |
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黄国强,熊向英,李洁,唐夏.溶解氧含量变动对褐牙鲆幼鱼生长及其相关生化指标的影响[J].广西科学院学报,2015,31(1):16-21. [点击复制]
- HUANG Guo-qiang,XIONG Xiang-ying,LI Jie,TANG Xia.Effects of Fluctuation of Dissolved Oxygen Content on the Growth, and Its Related Biochemical Pavameters in Juvenile Brown Flounder,Paralichthys olivaceus[J].Journal of Guangxi Academy of Sciences,2015,31(1):16-21. [点击复制]
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| 摘要: |
| [目的]以褐牙鲆(Paralichthys olivaceus)幼鱼为对象,研究其在不同溶解氧条件下的生长及生长指标的变化,探讨褐牙鲆幼鱼血浆激素的内分泌调节机制,为深入研究鱼类适应溶解氧变动环境的机制提供资料。[方法]采用实验生理生态学方法,研究水体溶解氧含量变动对褐牙鲆幼鱼生长、生长激素GH的分泌、类胰岛素生长因子IGF-Ⅰ的分泌、RNA/DNA比值以及糖原含量的影响。[结果]低溶氧(2 mg/L-S2、4 mg/L-S4)胁迫的10 d内,褐牙鲆幼鱼生长受到明显抑制,但低溶氧胁迫导致的生长减缓可在溶解氧含量恢复至对照组(7.5 mg/L-S7.5)水平后10 d内获得完全补偿。胁迫期间,血浆GH和IGF-Ⅰ的含量在不同处理组间没有显著差异,在恢复的第10天,S7.5的GH含量显著高于S2及S4,其他不同处理组的血浆GH和IGF-Ⅰ含量未出现显著差异。胁迫和恢复期间不同处理组的肝脏RNA/DNA比值均未出现显著差异。低溶氧处理的肌肉RNA/DNA比值在胁迫期间显著低于对照组,但在恢复生长的第20天显著高于对照组。胁迫结束时,低溶氧处理的肝糖原含量显著低于对照组,而在恢复期间的第10天和20天时高于对照组,随后恢复至对照组水平。不同处理组的肌糖原含量在胁迫和恢复期间均未出现显著差异。在恢复阶段的第10天,GH含量出现显著差异,其含量与生长速率呈负相关关系,而RNA/DNA含量在实验期间与褐牙鲆幼鱼的生长未表现出明显相关性。[结论]受短期低氧胁迫的褐牙鲆幼鱼能够在恢复正常溶氧后短期内获得完全补偿生长,但相关生化指标未表现出与生长变化的显著相关性。 |
| 关键词: 褐牙鲆 溶解氧 补偿生长 生长激素 类胰岛素生长因子Ⅰ |
| DOI： |
| 投稿时间：2014-10-10修订日期：2014-11-19 |
| 基金项目:国家自然科学基金项目(30600462)资助。 |
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| Effects of Fluctuation of Dissolved Oxygen Content on the Growth, and Its Related Biochemical Pavameters in Juvenile Brown Flounder,Paralichthys olivaceus |
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HUANG Guo-qiang1,2, XIONG Xiang-ying1, LI Jie2, TANG Xia2
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| (1.Guangxi Key Laboratory of Marine Biotechnology, Guangxi Institute of Oceanology, Beihai, Guangxi, 536000, China;2.Fisheries College, Ocean University of China, Qingdao, Shandong, 266003, China) |
| Abstract: |
| [Objective] To investigate the effects of oxygen content fluctuation on the growth and biochemical parameters of Paralichthys olivaceus, and discusses its endocrine regulation mechanism, which is important for studying the adapting mechanism to the changes of dissolved oxygen environment.[Methods] A physic-ecological experiment was conducted to investigate the effects of oxygen content fluctuation on the growth, GH, IGF-Ⅰ, RNA/DNA, and glycogen content of juvenile brown flounder, Paralichthys olivaceus.[Results] In the first 10 d, the growth of juvenile flounder in low dissolved oxygen content (2 mg/L-S2 and 4mg/L-S4) was obviously suppressed. But the growth suppression could be completely compensated in 10 d following the dissolved oxygen content switch to controlled level (about 7.5 mg/L-S7.5). No significant difference appeared in plasma GH and IGF-Ⅰ content in plasma in low DO stressing period and GH in S7.5 was significantly higher than S2 and S4 in the recovery period. No significant difference of GH and IGF-Ⅰ appeared in the following periods. No significant difference appeared in liver RNA/DNA through the all experiment, but the muscle RNA/DNA in S2 and S4 were significantly lower than S7.5 in low DO stressing period and were significantly higher than S7.5 at the end of the 20th d of recovery period. The liver glycogen content of S2 and S4 were significantly lower than S7.5 at the end of low DO stressing period. In the following 10th and 20th d of recovery period, liver glycogen content of S2 and S4 were significantly higher than S7.5 and then fell to the level of S7.5.No difference in glycogen content of muscle was observed through the experiment. Significant difference of GH in plasma appeared at the 10th d of recovery period, but the GH content was negatively related to the growth rate. No significant correlation between RNA/DNA and growth of juvenile flounder was found in the experiment.[Conclusion] Juvenile flounder experienced short periods stress of low dissolved oxygen content would achieved completely compensatory by growing in a short period with normal dissolved oxygen content. The biochemical parameters measured in experiment were not obviously related to the fluctuation of growth. |
| Key words: brown flounder dissolved oxygen compensatory growth growth hormone insuline-like growth factor I |
