| 引用本文： |
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潘丽霞,黎演明,竺利波.秀珍菇废渣中木质素降解酶系的研究[J].广西科学院学报,2014,30(2):101-103. [点击复制]
- PAN Li-xia,LI Yan-ming,ZHU Li-bo.Studies on Ligninases of Pleurotus Geesteranus[J].Journal of Guangxi Academy of Sciences,2014,30(2):101-103. [点击复制]
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| 摘要: |
| [目的]重点研究秀珍菇废渣中的木质素降解酶系。[方法]对秀珍菇废渣中可能存在的3种木质素降解酶:锰过氧化物酶(Manganese peroxidase,MnP)、木质素过氧化物酶(Lignin peroxidase,LiP)及漆酶(Laccase,Lac)进行活力测定,并对漆酶的最适pH值和最适温度进行考察。[结果]在秀珍菇废渣中未检测到锰过氧化物酶活力,检测到漆酶和木质素过氧化物酶活力。漆酶的最适pH值为6.2,最适温度为30℃。[结论]较一般食用菌不同,秀珍菇废渣中同时具有木质素过氧化物酶和漆酶活力,并且木质素过氧化物酶活力还较高。 |
| 关键词: 秀珍菇废渣 锰过氧化物酶 木质素过氧化物酶 漆酶 |
| DOI： |
| 投稿时间：2013-12-17修订日期：2014-01-20 |
| 基金项目:广西自然科学基金(2013GXNSFBA019089)资助。 |
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| Studies on Ligninases of Pleurotus Geesteranus |
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PAN Li-xia, LI Yan-ming, ZHU Li-bo
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| (Guangxi Academy of Sciences, State Key Laboratory of Non-food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China) |
| Abstract: |
| [Objective] This paper focuses on lignin degrading enzymes of pleurotus geesteranus.[Methods] Manganese peroxidase, lignin peroxidase and laccase from pleurotus geesteranus were detected. Optimal pH and temperature of laccase were also analyzed.[Results] The results showed that only manganese peroxidase and laccase were assayed in the pleurotus geesteranus residues and no lignin peroxidase were checked. Optimal pH and temperature of laccase were 6.2 and 30℃,respectively.[Conclusion] Different from normal mushroom, pleurotus geesteranus showed both lignin peroxidase and laccase activity. |
| Key words: pleurotus geesteranus residues manganese peroxidase lignin peroxidase laccase |
