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刘金平,于晶晶,牛福星,滕昆,覃晓丽,韦宇拓.地衣芽胞杆菌麦芽糖α-淀粉酶产麦芽糖特性研究[J].广西科学院学报,2013,29(3):171-175. [点击复制]
- LIU Jin-ping,YU Jing-jing,NIU Fu-xing,TENG Kun,QIN Xiao-li,WEI Yu-tuo.Studying on the Maltogenic α-amylase Property of the Production of Maltose from Bacillus licheniformis[J].Journal of Guangxi Academy of Sciences,2013,29(3):171-175. [点击复制]
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| 地衣芽胞杆菌麦芽糖α-淀粉酶产麦芽糖特性研究 |
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刘金平1,2, 于晶晶1,2, 牛福星1,2, 滕昆1,2, 覃晓丽1,2, 韦宇拓1,2
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| (1.广西大学生命科学与技术学院, 广西南宁 530005;2.亚热带农业生物资源保护与利用国家重点实验室, 广西南宁 530005) |
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| 摘要: |
| 为了开发新的生产麦芽糖浆的淀粉酶,在大肠杆菌中表达了一个地衣芽胞杆菌(Bacillus licheniformis)麦芽糖α-淀粉酶,并对该酶产麦芽糖的特性进行研究。结果显示,重组酶分子大小为65 kDa,以淀粉为底物的最适温度为45℃,最适pH值为6.5。以浓度为20%的可溶性淀粉为底物,加入106 U/g淀粉的地衣芽胞杆菌麦芽糖α-淀粉酶,反应48h,采用HPLC检测产物,产物中只有葡萄糖和麦芽糖,其中麦芽糖含量为52.21%,还原糖得率为72.1%;当加入1U/g淀粉的普鲁兰酶协同作用进行反应时,产物中麦芽糖的含量增加到57.16%,还原糖得率增加到92.5%。该酶在麦芽糖浆的工业生产上有较大的潜在应用价值。 |
| 关键词: 地衣芽胞杆菌 麦芽糖α-淀粉酶 麦芽糖 克隆表达 |
| DOI： |
| 投稿时间：2013-06-10修订日期：2013-06-18 |
| 基金项目:广西科学研究与技术开发计划(No.11107008-3),广西研究生教育创新计划项目(YCSZ2012027)资助。 |
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| Studying on the Maltogenic α-amylase Property of the Production of Maltose from Bacillus licheniformis |
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LIU Jin-ping1,2, YU Jing-jing1,2, NIU Fu-xing1,2, TENG Kun1,2, QIN Xiao-li1,2, WEI Yu-tuo1,2
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| (1.College of Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China;2.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning, Guangxi, 530005, China) |
| Abstract: |
| A maltogenic α-amylase from Bacillus licheniformis is cloned and expressed in E.coli.Enzymatic property and the production of maltose were studied for generating new amylase for maltose syrup.The recombinant enzyme molecular size was 65 kDa.The optimal activity for soluble starch hydrolysis was obtained at 60℃ and pH 6.6.The HPLC results showed that the hydrolysis products of 20% soluble starch by adding 106 U recombinant enzymes per gram starch for 48 hours were glucose and maltose and 52.21% of hydrolysates are maltose.The conversion rate was about 72.1%.Along with pullulanase (1U/g starch),the content of maltose reached 57.16% with 92.5% the conversion rate.The recombinant enzyme shows the great potential value in the production of maltose syrup industry. |
| Key words: Bacillus licheniformis maltogenic α-amylase maltose cloning and expression |
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