| 引用本文： |
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梁佳,张琳婧,卢日刚.高效毛细管电泳法测定牛奶中四环素类抗生素含量[J].广西科学院学报,2010,26(3):309-311,313. [点击复制]
- LIANG Jia,ZHANG Lin-jing,LU Ri-gang.Determination of Tetracycline Antibiotics in Milk by High Performance Capillary Electrophoresis[J].Journal of Guangxi Academy of Sciences,2010,26(3):309-311,313. [点击复制]
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| 摘要: |
| 采用固相萃取-毛细管电泳法(SPE-HPCE)同时检测牛奶中四环素、氯霉素、土霉素和金霉素的含量。以80mmol·L-1硼砂缓冲液为电泳介质,运行电压25kV,压力法进样5s;柱温25℃;检测波长200nm。结果表明,该方法能使4种抗生素在15 min内得到完全分离,检测限(μg·ml-1)分别为0.5,1.5,1.0,0.5,回收率分别为100.7%(RSD=1.3%),101.7%(RSD=0.6%),100.8%(RSD=1.5%),101.1%(RSD=0.7%).该方法简单、可靠,灵敏度高,适用于牛奶中四环素类抗生素残留量的检测。 |
| 关键词: 高效毛细管电泳法 固相萃取 四环素 牛奶 |
| DOI： |
| 投稿时间：2010-05-20 |
| 基金项目:广西自然科学基金项目[桂科自(0832109)]资助。 |
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| Determination of Tetracycline Antibiotics in Milk by High Performance Capillary Electrophoresis |
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LIANG Jia, ZHANG Lin-jing, LU Ri-gang
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| (Guangxi Institute for Food and Drug Control, Nanning, Guangxi, 530021, China) |
| Abstract: |
| A solid phases extraction-high performance capillary electrophoresis method was established for the assay of four tetracycline antibiotics in milk.The run buffer was boric solution(80mmol·L-1).The running voltage was 25kV and the detective wavelength was 200nm.Four samples were completely separated in 15 minutes. The detection limit(μg·ml-1) of the four compounds were 0.5,1.5,1.0,0.5, respectively.The average recoveries were 100.7%(RSD=1.3%),101.7%(RSD=0.6%),100.8%(RSD=1.5%),101.1%(RSD=0.7%),respectively.The method is simple,accurate,sensitive and can be applied to determine the tetracy cline antibiotics in milk. |
| Key words: HPCE (CZE) SPE tetracyclines milk |
