| 引用本文： |
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黄丽涵,曾永芳,徐远金.毛细管胶束电动色谱-质谱联用法同时测定心脑康胶囊中3种成分含量[J].广西科学院学报,2010,26(3):264-267. [点击复制]
- HUANG Li-han,ZENG Yong-fang,XU Yuan-jin.Determination of 3 Compositions in Xinnaokang Capsules by Micellar Electrokinetic Capillary Chromatography-Electrospray Ionization Mass Spectrometry[J].Journal of Guangxi Academy of Sciences,2010,26(3):264-267. [点击复制]
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| 摘要: |
| 采用胶束电动色谱-质谱联用法同时测定心脑康胶囊中的芍药苷、葛根素、大黄素含量。该方法以未涂层石英毛细管(80cm×50μm)为分离通道,以30 mmol/L月桂酸-90 mmol/L氨水(pH值9.0)为缓冲溶液,50%异丙醇(含1 mmol/L乙酸)为鞘液,工作电压为25kV。测定结果:各组分在18 min内达到完全分离,芍药苷、葛根素、大黄素的线性范围分别为0.20~200mg/L、0.50~500mg/L、0.020~300 mg/L,检出限分别为0.020mg/L、0.050mg/L、0.0060 mg/L。样品的加标回收率在96.5%~102%之间,相对标准偏差均小于3.1%。该方法简便、快速、准确可靠,已成功应用于心脑康胶囊中芍药苷、葛根素、大黄素的含量测定。 |
| 关键词: 毛细管胶束电动色谱 心脑康胶囊 芍药苷 葛根素 大黄素 |
| DOI： |
| 投稿时间：2010-05-01 |
| 基金项目:国家自然科学基金项目(20865001),广西自然科学基金项目(桂科自0832034)资助。 |
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| Determination of 3 Compositions in Xinnaokang Capsules by Micellar Electrokinetic Capillary Chromatography-Electrospray Ionization Mass Spectrometry |
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HUANG Li-han1, ZENG Yong-fang2, XU Yuan-jin1,2
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| (1.College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China;2.Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Nanning, Guangxi, 530004, China) |
| Abstract: |
| A MEKC-MS method for the simultaneous determination of paeoniflorin, puerarin and emodin in Xinnaokang Capsules was established.The samples were separated by an uncoated capillary(80cm×50μm) on the operating voltage of 25 kV using 30 mmol/L lauric acid-90 mmol/L ammonia mixture (containing 25% acetonitrile, pH value 9.0) as the running buffer and 50% 2-Propanol 50%water mixture (containing 1 mmol/L acetic acid) as the sheath liquid. Under the optimum conditions, the baseline separation of three compounds was achieved within 18 minutes. The detection limits were 0.020 mg/L, 0.050 mg/L, 0.0060 mg/L for paeoniflorin, puerarin and emodin, respectively. The average recoveries of the three components were from 96.5% to 102% with all RSD less than 3.1%. The method is simple, rapid, accurate and suitable for the quality control of Xinnaokang Capsules. |
| Key words: MEKC-ESI MS xinnaokang capsules paeoniflorin puerarin emodin |
