| 引用本文： |
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农军,吕仕军,段玉林,缪璐,昝川南,陀雄信.超高效液相色谱法测定露酒中的枸橼酸西地那非[J].广西科学院学报,2010,26(3):252-254. [点击复制]
- NONG Jun,LV Shi-jun,DUAN Yu-lin,MIAO Lu,ZAN Chuan-nan,TUO Xiong-xin.Detection of Sildenafil Citrate in the Liqueur by Ultra Performance Liquid Chromatography[J].Journal of Guangxi Academy of Sciences,2010,26(3):252-254. [点击复制]
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| 摘要: |
| 为了建立露酒中非法添加枸橼酸西地那非的一种快速、准确、灵敏高的专属性检测方法,采用反相超高效液相色谱法,以C18柱(2.1mm×100mm,1.7μm),柱温30℃,检测波长293.6nm,流动相为乙腈:水:冰醋酸:三乙胺(35:65:0.8:0.4;V/V),流速0.15ml/min的条件进行实验。结果枸橼酸西地那非在0.05~50μg/ml范围内线性关系良好,相关系数r=0.9998,平均回收率为98.93%,RSD=1.86%。建立的方法准确、可靠,可以用于露酒中枸橼酸西地那非的含量检测。 |
| 关键词: 超高效液相色谱 枸橼酸西地那非 露酒 |
| DOI： |
| 投稿时间：2010-06-06 |
| 基金项目: |
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| Detection of Sildenafil Citrate in the Liqueur by Ultra Performance Liquid Chromatography |
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NONG Jun, LV Shi-jun, DUAN Yu-lin, MIAO Lu, ZAN Chuan-nan, TUO Xiong-xin
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| (Guanxi Product Quality Supervision and Testing Institute, Nanning, Guangxi, 530022, China) |
| Abstract: |
| In order to estabish a quick,accurate,sensitive method for analysis of sildenafil citrate,which illegally added into the liqueur,reversed phase ultra performance liquid chromatography was used.The column was C18 2.1×100mm.Column temperature was 30℃.The mobile phase was acetonitrile:water:acetic acid:triethylamine(35:65:0.8:0.4;V/V) with flow rate 0.15 ml/min and the detection wavelength was 293.6 nm.The linear range of the calibration curve of sildenafil citrate were 0.05~50μg/ml (r=0.9998).The mean rates of recovery was 98.93% and the relative standard deviations (n=6) was 1.86%. The method is accurate, realiable that can be used to determine sildenfil citrate in the liqueur. |
| Key words: ultra performance liquid chromatography sildenafil citrate liqueur |
