| 引用本文： |
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李方青,曾维铭,周维官,陈业良,杨萍,韦平,毕朝斌,李振林.蛇源变形杆菌的分离鉴定[J].广西科学院学报,2000,(3):135-137,141. [点击复制]
- Li Fangqing,Zeng Weiming,Zhou Weiguan,Chen Yeliang,Yang Ping,Wei Ping,Bi Chaobin,Li Zhenlin.Isolation and Identification of Proteus from Diseased Snakes[J].Journal of Guangxi Academy of Sciences,2000,(3):135-137,141. [点击复制]
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| 摘要: |
| 取广西南宁、河池和柳州等地140条病死及濒死蛇的心血等作细菌培养。结果分离出细菌232株;分类鉴定发现79株为变形杆菌,占检出菌的34.1%;种的分类表明,63株为普通变形杆菌,16株为奇异变形杆菌;随机抽取其中5株进行毒素测定,均发现有肠毒素产生;做动物试验可致小白鼠死亡;做回归试验,可见蛇血带菌。说明,此变形杆菌具有一定的毒力,是造成蛇感染的常见菌之一,为人兽共患病的传染源,不可忽视。 |
| 关键词: 蛇 变形杆菌 分离 鉴定 肠毒素 |
| DOI： |
| 投稿时间：2000-04-13修订日期：2000-06-19 |
| 基金项目:广西自然科学基金项目(桂科自9912016)和广西科学院科技基金项目(桂科院研9808) |
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| Isolation and Identification of Proteus from Diseased Snakes |
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Li Fangqing, Zeng Weiming, Zhou Weiguan, Chen Yeliang, Yang Ping, Wei Ping, Bi Chaobin, Li Zhenlin
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| (Laboratory of Microbiology, Guangxi Health Administration College, Nanning, 530021) |
| Abstract: |
| 232 bacteria were isolated from the heart blood samples of 140 die of illness or dying snakes collected in Nanning,Hechi and Liuzhou of Guangxi; The results of identification and classification revealed that 79 bacterial isolates belonged to the genus of Proteus, among them P. vulgaria was the dominant species with 63 isolates,and P. mirabilis with 16 isolates. Toxin test with 5 randomly chosen isolates showed positive. Pathogenicity tests with mouce and snake resulted in death and bacteremia respectively.The results demonstrated that proteus as a conditioned pathogen is an important agent in zoonosis. |
| Key words: snake proteus isolation identification enterotoxin |
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