| 引用本文： |
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周素芳,秦雪,梁谷,吴耀生,周德义.用酶标凝集素检测胃液中的胃蛋白酶[J].广西科学院学报,1997,(3):31-33. [点击复制]
- Zhou Sufang,Qin Xue,Liang Gu,Wu Yaosheng,Zhou Deyi.Gastric Juice Pepsin Assay by Enzyme-labeled Lectin[J].Journal of Guangxi Academy of Sciences,1997,(3):31-33. [点击复制]
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| 摘要: |
| 采用辣根过氧化物酶(HRP)标记ALL,通过夹心酶联法检测各种胃病患者胃液中的胃蛋白酶。结果:胃蛋白酶在正常组(15例)、胃炎组(28例)、十二指肠溃疡组(25例)、胃溃疡组(25例)及胃癌组(13例)中的含量无显著性差异。但胃液pH值≥5的胃蛋白酶含量明显高于pH值<4的酶含量。表明胃粘膜屏障的局部缺陷使之对胃蛋白酶抵抗能力下降,可能与消化性溃疡的发生关系更为密切。 |
| 关键词: 红桂木凝集素 酶联免疫测定 胃液 胃蛋白酶 |
| DOI： |
| 投稿时间：1997-06-26 |
| 基金项目:国家自然科学基金(编号39260023) |
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| Gastric Juice Pepsin Assay by Enzyme-labeled Lectin |
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Zhou Sufang, Qin Xue, Liang Gu, Wu Yaosheng, Zhou Deyi
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| (Department of Biochemistry, Guangxi Medical University, Nanning, 530021) |
| Abstract: |
| The pepsin contents of gastric juice were determined by ALL-HRP sandwich assay.Results:There were no significant differences in the pepsin contents among the normal controls and patients with various gastric diseases including gastritis,duodenal ulcer,peptic ulcer and carcinoma of stomach.But the pepsin contents of the gastric secretions with pH ≥ 5 were significantly higher than those with pH<4. |
| Key words: Artocarpus lingnanesis lectin enzyme linked immunoassay gastric juice pepsin |
