| 引用本文： |
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何斌源,梁士楚,凌俊文.红树植物叶片叶绿素提取方法比较及其活体测定[J].广西科学院学报,1993,(2):77-81. [点击复制]
- He Binyuan,Liang Shichu,Ling Junwen.Comparision of Extraction Methods Used to Determine Chlorophyl Content in Mangrove Leaves and Alive Leaf Determination[J].Journal of Guangxi Academy of Sciences,1993,(2):77-81. [点击复制]
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| 摘要: |
| 本文采用混合液法测定了五种红树植物叶片的叶绿素含量,并摸索了提取过程中混合液的不同配比和提取的时间。对相同的材料用Arnon法和混合液法进行对照测定,结果表明Arnon法比混合液法提取红树植物叶片叶绿素效果好.通过Arnon法和活体叶绿素仪的平行测定,分别建立了四种红树植物叶片的活体测定值D与叶绿素含量(mg/cm2)的回归方程,由此得出快速测定红树植物叶片叶绿素含量的简便方法。 |
| 关键词: 红树植物 叶绿素 提取方法 活体测定 |
| DOI： |
| 投稿时间：1993-08-17 |
| 基金项目: |
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| Comparision of Extraction Methods Used to Determine Chlorophyl Content in Mangrove Leaves and Alive Leaf Determination |
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He Binyuan1, Liang Shichu1, Ling Junwen2
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| (1.Guangxi Mangrove Research Center, Beihai 536000;2.Department of Biology, Guangxi Normal University, Guilin 541004) |
| Abstract: |
| This paper deals with the methods of extracting chlorophyl from mangrove leaves.We apply mixed-solution method to extract chlorophyl of five mangrove species from Dandou and Daguansha,Beihai,and grope for varied solution proportions and extracting time during the extraction process.In comparing the mixed-solution method with Arnon method,We abtain a conclusion that Arnon method is more fit for chlorophyl extraction from mangrove plants.From parallel determination of Arnon method and HYL-1 chlorophyl instrument,the four mangrove species regression equations about chlorophyl content (mg/cm2) and value D are given out. |
| Key words: mangrove plant chlorophyl extraction method |
