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  • 李春瑶,黎泳珊,陈高琼,何爱民,黄玉环.甘蔗愈伤组织耐盐性研究:耐盐愈伤组织的筛选及脯氨酸、还原糖含量变化[J].广西科学院学报,1992,(2):59-63.    [点击复制]
  • Li Chunyao,Li Yongshan,Chen Gaoqiong,He Aiming,Huang Yuhuan.Study on Salt-Tolerance of Sugarcane Callus——Selection of Salt-Tolerance Callus and Changes of Proline, Reducing Sugar Level[J].Journal of Guangxi Academy of Sciences,1992,(2):59-63.   [点击复制]
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甘蔗愈伤组织耐盐性研究:耐盐愈伤组织的筛选及脯氨酸、还原糖含量变化
李春瑶, 黎泳珊, 陈高琼, 何爱民, 黄玉环
0
(广西师范大学生物系细胞研究室, 桂林 541004)
摘要:
在附加不同浓度NaCl及2.4-D 2mg/L的MS培养基上,甘蔗愈伤组织表现出不同的生长效应。培养基中NaCl浓度低时(1%以下),甘蔗愈伤组织表现为适应性生长;而在高NaCl浓度时(2%以上),愈伤组织生长表现出盐害特征。脯氨酸、还原糖测定结果显示,在盐适应情况下,甘蔗愈伤组织中的脯氨酸含量较高,还原糖含量较低;在盐害情况下则相反。
关键词:  甘蔗愈伤组织  NaCl  脯畅酸  还原糖
DOI:
投稿时间:1991-10-19
基金项目:
Study on Salt-Tolerance of Sugarcane Callus——Selection of Salt-Tolerance Callus and Changes of Proline, Reducing Sugar Level
Li Chunyao, Li Yongshan, Chen Gaoqiong, He Aiming, Huang Yuhuan
(Department of Biology, Guangxi Normal University, Guilin 541004)
Abstract:
On MS basal medium supplemented with 2 mg/L 2.4-D and different concentrations of NaCl, sugarcane callus shows different growth effects. As he concentrations of NaCl in medium are low (<1%), the callus appears in adaptive growth and as the concentrations of NaCl are high (>2%), the callus is to be salt-injured. The levels of proline and reducing sugar of callus were measured and the results shows that the salt-adapted callus has a high level proline and low level reducing sugar, the salt-injured callus has a low level proline and high level reducing sugar.
Key words:  Sugarcane callus  NaCl  Proline  Reducing sugar

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