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| 广西克氏原鳌虾细菌流行病学调查及分离菌耐药分析 |
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陈婷婷1, 卢冰霞1, 许艺兰1, 周英宁1, 梁家幸1, 黄广杰2, 许心婷1, 全琛宇1, 曾家家3, 江新华3, 陈忠伟1, 何 颖1
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| (1.广西壮族自治区兽医研究所广西兽医生物技术重点实验室/农业农村部中国广西-东盟跨境动物疫病防控重点实验室;2.贵港市水产技术推广站;3.玉林市水产技术推广站) |
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| 摘要: |
| 对广西地区克氏原鳌虾常见细菌性病原进行流行性调查和耐药情况分析,为此类病原导致的疾病进行有效综合防控提供数据支持。采集广西不同地区养殖场和市场患病克氏原螯虾作为样本,通过细菌性病原的分离鉴定和分离菌药敏试验,分析克氏原螯虾细菌感染种类、分布情况和耐药特点。病原分离鉴定结果显示,本研究共分离纯化细菌364株,总分离率为79.8%,主要为革兰氏阴性菌,其中弗氏柠檬酸杆菌、布氏柠檬酸杆菌和豚鼠气单胞菌占比最高,分别为29.9%、10.4%和9.9%；分离菌有明显的地域特征,以上三种细菌在广西克氏原螯虾的主产地来宾、贵港、南宁分布最广,分离率在4.0%~47.6%；这3种菌主要侵害克氏原鳌虾的肝胰腺和肠道；分离菌对β-内酰胺类(青霉素G、头孢拉定)、大环内酯类(阿奇霉素)、多肽类(多粘菌素B)耐药,耐药率在22.2%~100.0%,对林可霉素、磷霉素的敏感率在55.6%~100.0%；广西地区克氏原螯虾养殖场应针对弗氏柠檬酸杆菌、布氏柠檬酸杆菌和豚鼠气单胞菌三种主要细菌性疫病做好净化水质、临床推荐用药来进行有效综合防控。 |
| 关键词: 克氏原鳌虾 流行病学调查 细菌分离 药敏试验 |
| DOI： |
| 投稿时间：2024-01-03修订日期：2024-03-21 |
| 基金项目:广西重点研发计划(桂科AB21076008,桂科AB20297059)；广西青年科学基金(2023GXNSFBA026295)；广西农业科技自筹经费项目(Z202226,Z202224) |
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| Epidemiology and Antibiotic Resistance of Bacteria Isolated from Procambarus Clarkii in Guangxi, China |
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CHEN Ting-ting1, LU Bing-xia1, XU Yi-lan1, ZHOU Yin-ning1, LIANG Jia-xing1, HUANG Guang-jie2, XU Xin-ting1, QUAN Chen-yu1, JIANG Xin-hua3, ZENG Jia-jia3, CHEN Zhon-gwei1, HE Yin1
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| (1.Guangxi Key Laboratory of Veterinary Biotechnology Guangxi Veterinary Research Institute/ Key Laboratory of ChinaGuangxi-ASEAN Cross-border Animal Disease Prevention and Control,Ministry of Agriculture and Rural Affairs of China,Nanning;2.Guigang Aquaculture Technology Extending Station,Guigang;3.Yulin Aquaculture Technology Extending Station,Yulin,,China) |
| Abstract: |
| Prevalence survey and drug resistance analysis of common bacterial pathogens of Cambarus Clarkii in Guangxi area were performed. And that provided data support for effective integrated prevention and control of diseases caused by such pathogens. Diseased Cambarus Clarkii from different farms and markets of Guangxi were collected as samples. These samples were subjected to isolation and identification of bacterial pathogens, drug sensitivity testing, and then analyse the species, distribution and drug resistance of bacterial infection. The results of pathogen isolation and identification showed that a total of 364 bacterial strains were isolated and purified in this study, with an overall isolation rate of 79.8%. It was found that the isolates were mainly Gram-negative bacteria, among which Citrobacter freundii, Citrobter braakii and Aeromonas caviae accounted for the highest percentage, 29.9%, 10.4% and 9.9%, respectively. These three bacteria mainly attack the hepatopancreas and intestine of Procambarus clarkii, causing vacuolation of liver cells and swelling of intestinal glands. The isolated bacteria had obvious geographical characteristics, and the above three bacteria were widely distributed in Laibin, Guigang and Nanning with isolation rates ranging from 4% to 47.6%. And the three place above were the main production area of Procambarus clarkii in Guangxi. Due to the widespread drug resistance of pathogenic bacteria to conventional antibiotics, clinical drug use should be combined with the type of pathogenic bacteria and drug sensitivity test results. |
| Key words: Procambarus clarkii Epidemiological Investigation Isolation and identification drug sensitivity test |
