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  • 秦艳,申乃坤,莫勇生,朱婧,王青艳,廖思明.黄浆水与木薯粉混合发酵高浓度乙醇的发酵条件[J].广西科学,2012,19(3):249-252.    [点击复制]
  • QIN Yan,SHEN Nai-kun,MO Yong-sheng,ZHU Jing,WANG Qing-yan,LIAO Si-ming.Techniques for Fermentating High-concentration Ethanol from Mixing Yellow Serofluid and Cassava[J].Guangxi Sciences,2012,19(3):249-252.   [点击复制]
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黄浆水与木薯粉混合发酵高浓度乙醇的发酵条件
秦艳1, 申乃坤1, 莫勇生2, 朱婧1, 王青艳1, 廖思明3
0
(1.广西科学院国家非粮生物质能源工程技术研究中心, 广西南宁 530007;2.广西科学院生物研究所, 广西南宁 530007;3.广西红树林研究中心, 广西北海 536000)
摘要:
以黄浆水与木薯粉混合同步糖化发酵高浓度乙醇,应用Plackett-Burman设计对底物浓度、尿素、糖化酶、液化酶、MgSO4、pH值、CaCl2、KH2PO4 8个因素进行2水平12次试验,研究发酵的最佳条件。结果发现,底物浓度、尿素、糖化酶和KH2PO4的添加量是影响发酵的重要因素,最佳发酵条件是底物浓度38%、尿素0.15%、糖化酶1.45AGU/g、KH2PO4 0.07%。在最佳发酵条件下,乙醇浓度达到17.21%(V/V)。
关键词:  黄浆水  木薯粉  同步糖化发酵  Plackett-Burman设计  乙醇
DOI:
投稿时间:2012-01-30修订日期:2012-03-16
基金项目:广西科学研究与技术开发计划项目(桂科重12118004-3);广西区科学研究与技术开发课题(桂科攻1099070,桂科攻11107008-4);广西科学院基金项目(No.10YJ25SW07)资助。
Techniques for Fermentating High-concentration Ethanol from Mixing Yellow Serofluid and Cassava
QIN Yan1, SHEN Nai-kun1, MO Yong-sheng2, ZHU Jing1, WANG Qing-yan1, LIAO Si-ming3
(1.National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.Institute of Biology of Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;3.Guangxi Mangrove Research Center, Beihai, Guangxi, 536000, China)
Abstract:
The conditions of simultaneous saccharification and fermentation by mixing yellow serofluid and cassava flour were investigated for producing high-concentration ethanol. The important parameters were screened by Plackett-Burman design, and subsequently the path of steepest ascent was used to approach to the biggest region of ethanol production. Finally, the optimum values of parameters were obtained by orthogonal tests.The experimental results showed the optimum technical conditions as follows:Substrate concentration 38%, CO (NH2)2 0.15%, Dextrozyme 1.45 AGU/g substrate, KH2PO4 0.07%, under which the ethanol reached 17.21% (V/V) after 48 h fermentation at 32℃ and 100 r/min.
Key words:  yellow serofluid  cassava flour  simultaneous saccharification and fermentation  Plackett-Burman design  ethanol

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