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张耿思,李海艳,傅春青,苏志维,周冬梅,高程海,刘永宏,徐新亚.广西北海竹林盐场烟曲霉GXIMD 00544的次级代谢产物研究[J].广西科学,2025,32(2):322-329. [点击复制]
- ZHANG Gengsi,LI Haiyan,FU Chunqing,SU Zhiwei,ZHOU Dongmei,GAO Chenghai,LIU Yonghong,XU Xinya.Secondary Metabolites of a Beihai Zhulin Saltern-Derived Fungus Aspergillus fumigatus GXIMD 00544[J].Guangxi Sciences,2025,32(2):322-329. [点击复制]
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| 广西北海竹林盐场烟曲霉GXIMD 00544的次级代谢产物研究 |
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张耿思, 李海艳, 傅春青, 苏志维, 周冬梅, 高程海, 刘永宏, 徐新亚
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| (广西中医药大学海洋药物研究院, 广西海洋药物重点实验室, 广西 南宁 530200) |
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| 摘要: |
| 为研究一株广西北海竹林盐场来源真菌烟曲霉(Asperigillus fumigatus) GXIMD 00544中的次级代谢产物并考察其抗菌活性，本研究采用多种柱色谱分离并结合波谱数据，分析确定次级代谢产物的化合结构，再进一步采用96孔板法测定其抗菌活性。结果表明，该菌株的乙酸乙酯萃取物分离后可得到11个化合物，包括4个烟曲霉酸类化合物(1-4)和7个酚酸类化合物(5-11)，分别鉴定为maunakeanolic acid A(1)、6-deacytyl-1,2-dihydrohelvolic acid(2)、helvolic acid(烟曲霉酸，3)、helvolinic acid(4)、8'-O-methylasterric acid(5)、methylated asterric acid(6)、dimethyl 2,3'-dimethylosoate(7)、dimethylsoluchrin(8)、sulochrin(9)、monomethylsulochrin(10)、trypacidin(11)。其中，化合物1、3、4对人类致病菌耐甲氧西林金黄色葡萄球菌(Methicillin-Resistant Staphylococcus aureus，MRSA)表现出较强的抑制作用，最小抑菌浓度(Minimal Inhibitory Concentration,MIC)分别为5.12、5.68、2.63 μg·mL-1;对海洋污损细菌轮虫弧菌(Vibrio rotiferianus)也有较强的抑制作用,最小抑菌浓度分别为6.40、7.10、13.15 μg·mL-1；对海洋污损细菌海弧菌(V.pelagius)表现出抑制作用。烟曲霉酸类化合物1-4均具有较强的广谱抗菌活性。 |
| 关键词: 盐场来源真菌 烟曲霉 烟曲霉酸 酚酸衍生物 抗菌 |
| DOI：10.13656/j.cnki.gxkx.20250624.013 |
| 投稿时间：2023-12-11修订日期：2023-12-22 |
| 基金项目:国家自然科学基金项目(42066006;U20A20101);广西创新研究团队项目(2020GXNSFGA297002);广西自然科学基金-粤桂联合基金项目(2021GXNSFDA075010);广西八桂学者专项经费项目(05019055);广西中医药大学桂派杏林拔尖人才资助项目(2022C011);中药学广西一流学科项目(桂教科研〔2022〕1号)和广西研究生教育创新计划项目(JGY2022181;YCSW2023381)资助。 |
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| Secondary Metabolites of a Beihai Zhulin Saltern-Derived Fungus Aspergillus fumigatus GXIMD 00544 |
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ZHANG Gengsi, LI Haiyan, FU Chunqing, SU Zhiwei, ZHOU Dongmei, GAO Chenghai, LIU Yonghong, XU Xinya
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| (Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China) |
| Abstract: |
| This study aims to investigate the secondary metabolites of a Beihai Zhulin saltern-derived fungus Aspergillus fumigatus GXIMD 00544 and explore their antibacterial activities.Multiple column chromatography methods and the spectral data were employed to identify the chemical structures of the secondary metabolites.Furthermore,the 96-well plate method was employed to determine the antibacterial activities of these metabolites.Eleven compounds,helvolic acids (1-4) and phenolic acid derivatives (5-11),were separated from the ethyl acetate extract of this strain.These compounds were identified as maunakeanolic acid A (1),6-deacetyl-1,2-dihydrohelvolic acid (2),helvolic acid(3),helvolinic acid (4),8'-O -methylasterric acid (5),methylated asterric acid (6),dimethyl-2,3'-dimethylosoate(7),dimethylsulochrin (8),sulochrin (9),monomethylsulochrin(10),and trypacidin(11).Among them,compounds 1,3,and 4 showed strong inhibitory effects on the human pathogenic bacterium Methicillin-Resistant Staphylococcus aureus (MRSA),with minimum inhibitory concentrations (MIC) of 5.12,5.68,and 2.63 μg·mL-1,respectively.Furthermore,they exhibited strong inhibitory effects on the marine biofouling bacterium Vibrio rotiferianus,with MIC of 6.40,7.10,and 13.15 μg·mL-1,respectively.In addition,the three compounds demonstrated inhibitory effects on the marine biofouling bacterium V.pelagius.The helvolic acids 1-4 all demonstrated strong broad-spectrum antibacterial activities. |
| Key words: saltern-derived fungus Aspergillus fumigatus helvolic acids phenolic acid derivatives antibacterial |
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