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王梦芹,帖青清,黄晓雯,杨斌,刘永宏,李云秋.北部湾珊瑚共附生真菌Cladosporium sp.SCSIO41206次级代谢产物研究[J].广西科学,2025,32(2):313-321. [点击复制]
- WANG Mengqin,TIE Qingqing,HUANG Xiaowen,YANG Bin,LIU Yonghong,LI Yunqiu.Secondary Metabolites of Cladosporium sp.SCSIO41206,a Symbiotic Epiphytic Fungus of Coral in the Beibu Gulf[J].Guangxi Sciences,2025,32(2):313-321. [点击复制]
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| 北部湾珊瑚共附生真菌Cladosporium sp.SCSIO41206次级代谢产物研究 |
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王梦芹1, 帖青清1, 黄晓雯1, 杨斌2, 刘永宏2, 李云秋1
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| (1.桂林医学院, 广西 桂林 541004;2.中国科学院南海海洋研究所, 热带海洋生物资源与生态重点实验室, 广东 广州 510301) |
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| 摘要: |
| 本研究基于单菌多次级代谢产物(OSMAC)策略对一株北部湾珊瑚共附生真菌Cladosporium sp.SCSIO41206中的次级代谢产物进行探究，采用文献数据比对的方法对化合物结构进行鉴定，并对化合物进行酶抑制活性和抗菌活性筛选。结果表明，共分离鉴定出11个化合物：3-isobutylhexahydropyrrolo(1,2-a) pyrazine-1,4-dione(1)、3-benzyl-7-hydroxyhexahydropyrrolo(1,2-a) pyrazine-1,4-dione(2)、环(L-丙氨酸-L-4-羟基-脯氨酸)二肽(3)、环(L-脯氨酸-甘氨酸)二肽(4)、cyclo(D)-Pro-(D)-Ile(5)、环(L-苯丙-甘)二肽(6)、cyclo-(4-S-hydroxy-R-proline-R-isoleucine)(7)、N-phenethylacetamide(8)、N-hydroxy-2-(hydroxyimino)-4-methylpentanamide(9)、quinolactacin A1(10)、quinolactacin A2(11)，化合物1-11均是首次从北部湾珊瑚来源的枝孢菌属(Cladosporium sp.)中分离获得。活性测试结果表明，化合物2-4、8、10和11对乙酰胆碱酯酶有弱的抑制活性；化合物4、8对胰脂肪酶具有较弱的抑制活性，化合物1-3、5、7、9-11对胰脂肪酶具有弱的抑制活性，化合物6对胰脂肪酶有中等的抑制活性；未筛查到相关的抗菌活性。本研究结果揭示了北部湾枝孢菌属真菌次级代谢产物的多样性特征，可为抗肥胖药物的研发奠定重要的理论基础。 |
| 关键词: 枝孢菌属 次级代谢产物 分离纯化 结构鉴定 酶抑制活性 |
| DOI：10.13656/j.cnki.gxkx.20231116.001 |
| 投稿时间：2023-10-26修订日期：2023-11-11 |
| 基金项目:国家自然科学基金项目(81860626)资助。 |
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| Secondary Metabolites of Cladosporium sp.SCSIO41206,a Symbiotic Epiphytic Fungus of Coral in the Beibu Gulf |
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WANG Mengqin1, TIE Qingqing1, HUANG Xiaowen1, YANG Bin2, LIU Yonghong2, LI Yunqiu1
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| (1.Guilin Medical University, Guilin, Guangxi, 541004, China;2.Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanography, Chinese Academy of Sciences, Guangzhou, Guangdong, 510301, China) |
| Abstract: |
| The One Strain Many Compounds (OSMAC) strategy was employed to explore the secondary metabolites of Cladosporium sp.SCSIO41206,a symbiotic epiphytic fungus of coral in the Beibu Gulf.The compound structures were identified by comparison with literature data.Furthermore,the compounds with enzyme inhibition activity and antibacterial activity were screened.The results showed that a total of 11 compounds were isolated and identified,namely 3-isobutylhexahydropyrrolo (1,2-a) pyrazine-1,4-dione (1),3-benzyl-7-hydroxyhexahydropyrolo (1,2-a) pyrazine-1,4-dione (2),cyclic (L -alanine-L -4-hydroxyproline) dipeptide (3),cyclic (L -proline glycine) dipeptide (4),cyclo (D)-Pro-(D)-Ile (5),cyclic (L -phenylpropyl glyceride) dipeptide (6),cyclo-(4-S -hydroxy-R -proline-R -isoleucine)(7),N -phenethylacetamide (8),N -hydroxy-2-(hydroxyimino)-4-methylpentamide (9),quinolactacin A1 (10),and quinolactacin A2 (11).Compounds 1-11 were isolated for the first time from Cladosporium sp..The activity test results showed that compounds 2-4,8,10,and 11 had weak inhibitory activity against acetylcholinesterase.Compounds 4 and 8 had weaker inhibitory activity against pancreatic lipase,and compounds 1-3,5,7,and 9-11 had weak inhibitory activity against pancreatic lipase.However,compound 6 had moderate inhibitory activity against pancreatic lipase.No relevant antibacterial activity was detected.The findings enrich the diversity of secondary metabolites of Cladosporium sp. from the Beibu Gulf and lay a theoretical foundation for the discovery of active lead compounds for the treatment of obesity. |
| Key words: Cladosporium sp. secondary metabolites separation and purification structural identification inhibitory activity against enzymes |
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