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万凌云,冀晓雯,潘丽梅,宋利沙,梁文静,杨翠红,闫志刚,张占江,韦树根.钩藤的茎钩与花序梗在不同发育时期的内源激素变化与分析[J].广西科学,2025,32(2):255-265. [点击复制]
- WAN Lingyun,JI Xiaowen,PAN Limei,SONG Lisha,LIANG Wenjing,YANG Cuihong,YAN Zhigang,ZHANG Zhanjiang,WEI Shugen.Variations in Content of Endogenous Phytohormones in the Hook and Peduncle of Uncaria rhynchophylla at Different Developmental Stages[J].Guangxi Sciences,2025,32(2):255-265. [点击复制]
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| 钩藤的茎钩与花序梗在不同发育时期的内源激素变化与分析 |
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万凌云1,2,3, 冀晓雯4, 潘丽梅1,2,3, 宋利沙1,2,3, 梁文静1,2,3, 杨翠红1,2,3, 闫志刚1,2,3, 张占江1,2,3,4, 韦树根1,2,3
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| (1.广西壮族自治区药用植物园, 广西道地药材高品质形成与应用重点实验室, 广西 南宁 530023;2.广西壮族自治区药用植物园, 广西中药材良种繁育技术创新中心, 广西 南宁 530023;3.广西壮族自治区药用植物园, 国家中医药传承创新中心, 广西 南宁 530023;4.广西壮族自治区药用植物园, 西南濒危药材资源开发国家工程研究中心, 广西 南宁 530023) |
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| 摘要: |
| 为探究内源激素在钩藤(Uncaria rhynchophylla)茎钩、花序梗形成和发育过程中的作用，本研究采用液相色谱-质谱(LC-MS)联用方法测定茎钩和花序梗发育过程中内源激素的动态变化并分析其规律。结果表明，钩藤茎钩和花序梗发育过程中内源激素含量及变化趋势差异明显。细胞分裂素类iP、iPR、tZ、cZ在钩藤茎钩和花序梗发育过程中的变化趋势近乎是相反的，在花序梗中呈上升趋势，在茎钩中则呈下降趋势。经相互作用网络分析，tZ、cZ是茎钩发育过程中节点内源激素。因此，细胞分裂素类是调控钩藤茎钩和花序梗差异发育的关键内源激素；赤霉素(Gibberellic Acid，GA)类(GA1、GA3)、脱落酸类(Abscisic Acid，ABA)在钩藤花序梗发育过程中呈上升趋势，在盛花期达到最大值，它们是钩藤花序梗发育过程中的节点内源激素，表明钩藤花序梗中高水平的GA1、GA3、ABA有利于钩藤花器官的发育与成熟。本研究揭示了内源激素在钩藤茎钩和花序梗差异发育中的关键作用，为生产实践中精准调控钩藤茎钩和花序梗的发育提供了理论依据。 |
| 关键词: 钩藤 内源激素 茎钩 花序梗 同源器官 |
| DOI：10.13656/j.cnki.gxkx.20250430.001 |
| 投稿时间：2024-06-11修订日期：2024-08-04 |
| 基金项目:广西科技重大专项项目(桂科AA22096021);国家自然科学基金项目(82360753);广西自然科学基金项目(2019GXNSFBA185026);广西特色药材关键技术研究推广项目(GZKJ2314);广西药用植物园科研基金项目(桂药基202013)和广西中药材品质创新研究团队项目(GZKJ2305)资助。 |
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| Variations in Content of Endogenous Phytohormones in the Hook and Peduncle of Uncaria rhynchophylla at Different Developmental Stages |
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WAN Lingyun1,2,3, JI Xiaowen4, PAN Limei1,2,3, SONG Lisha1,2,3, LIANG Wenjing1,2,3, YANG Cuihong1,2,3, YAN Zhigang1,2,3, ZHANG Zhanjiang1,2,3,4, WEI Shugen1,2,3
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| (1.College of Tourism and Landscape Architecture, Guilin University of Technology, Guilin, Guangxi, 541006, China;2.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China;3.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Normal University, Guilin, Guangxi, 541006, China;4.College of Pharmacy, Guilin Medical University, Guilin, Guangxi, 541004, China) |
| Abstract: |
| To explore the functions of endogenous phytohormones in the formation and development of the hook and peduncle of Uncaria rhynchophylla,this study employed Liquid Chromatography-Mass Spectrometry (LC-MS) to unveil the dynamic changes of endogenous phytohormones in the hook and peduncle of U.rhynchophylla at different developmental stages and then analyzed their patterns.The results showed that the content and variation trends of endogenous phytohormones in the hook and peduncle of U.rhynchophylla at different developmental stages showed significant differences.The content of iP,iPR,tZ,and cZ presented almost opposite variation trends in the hook and peduncle of U.rhynchophylla at different developmental stages,showing an upward trend in the peduncle but a downward trend in the hook.The interaction network analysis suggested that tZ and cZ were the core endogenous phytohormones during the hook development process.These results indicated that cytokinins were the key endogenous phytohormones regulating the differential development of the hook and peduncle of U.rhynchophylla.Gibberellic Acid (GA)(GA1,GA3),and Abscisic Acid(ABA)showed an upward trend during the development process of the peduncle of U.rhynchophylla and reached the maximum value at the full flowering stage.They were also the node endogenous phytohormones during the development process of the peduncle of U.rhynchophylla.This result indicated that high levels of GA1,GA3,and ABA in the peduncle were conducive to the development and maturity of U.rhynchophylla floral organs.In conclusion,endogenous phytohormones play a key role in the differential developmental patterns of the hook and peduncle of U.rhynchophylla.The results provide a theoretical basis for the precise regulation of the development of the hook and peduncle of U.rhynchophylla in production practice. |
| Key words: Uncaria rhynchophylla endogenous phytohormone hook peduncle homologous organ |
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