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周施雨,钱志萍,曹建国,戴锡玲.阴地蕨提取物成分分析及其抑菌抗肿瘤活性研究[J].广西科学,2025,32(5):1042-1049. [点击复制]
- ZHOU Shiyu,QIAN Zhiping,CAO Jianguo,DAI Xiling.Composition and Antibacterial and Antitumor Effects of the Extract of Sceptridium ternatum
[J].Guangxi Sciences,2025,32(5):1042-1049. [点击复制]
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| 摘要: |
| 为明确传统药用蕨类植物阴地蕨(Sceptridium ternatum)的生物活性和有效成分，本研究采用乙醇浸提、旋转蒸发、有机物萃取、浓缩等方法对其进行有机相提取，同时对石油醚部分和乙酸乙酯部分进行抑菌及抗肿瘤活性测定；然后，采用葡聚糖凝胶柱层析分段、Mitsubishi Chemical Industries(MCI)树脂去除色素、薄层色谱(TLC)分离及初步检测、重结晶提纯等方法对阴地蕨提取物进行成分分离，并使用波谱、质谱等方法进行化合物的结构鉴定。结果表明：(1)石油醚部分和乙酸乙酯部分对大肠杆菌(Escherichia coli)和金黄色葡萄球菌(Staphylococcus aureus)均具有抑菌活性，且石油醚部分的抑菌效果优于乙酸乙酯部分。(2)石油醚部分和乙酸乙酯部分对人肺癌(A-549)细胞和人宫颈癌(HeLa)细胞的增殖有一定的抑制作用，其中石油醚部分的抑制效果明显优于乙酸乙酯部分，且呈现较为明显的给药浓度依赖性。(3)石油醚部分对A-549细胞的半抑制浓度(IC50)为213.873 5 mg·L-1,对Hela细胞的IC50为365.626 4 mg·L-1。(4)从阴地蕨提取物中共分离出9种单体化合物，包括3种已报道化合物(分别为木犀草素、胡萝卜苷、β-谷甾醇)以及6种从阴地蕨中首次分离得到的化合物(分别为藿烷-22,29-二醇单乙酸酯、新松香酸、2',3'-二羟基十五烷酸丙酯、山嵛酸环氧乙烷甲酯、对羟基苯甲酸、苯十三酸甲酯)。本研究对阐明阴地蕨的成分及其生物活性具有一定的参考价值。 |
| 关键词: 阴地蕨 化合物 分离鉴定 抑菌作用 抗肿瘤活性 |
| DOI：10.13656/j.cnki.gxkx.20251208.019 |
| 投稿时间：2025-08-10修订日期：2025-09-12 |
| 基金项目:上海市绿化和市容管理局科学技术项目(G242415)资助。 |
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Composition and Antibacterial and Antitumor Effects of the Extract of Sceptridium ternatum
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ZHOU Shiyu1,2, QIAN Zhiping1, CAO Jianguo1, DAI Xiling1
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| (1.Shanghai Normal University, Shanghai, 200234, China;2.Shanghai General Hospital, Shanghai, 200080, China) |
| Abstract: |
| This study aimed to clarify the biological activities and active components of the traditional medicinal fern Sceptridium ternatum.Methods such as ethanol extraction,rotary evaporation,organic solvent extraction,and concentration were employed to obtain organic phase extracts.The antibacterial and antitumor activities of the petroleum ether and ethyl acetate fractions were evaluated.Component separation of the S.ternatum extract was performed through dextran gel column chromatography fractionation,pigment removal via Mitsubishi Chemical Industries (MCI) resin,Thin-Layer Chromatography (TLC) separation and preliminary detection,and recrystallization for purification.Compound structures were identified based on spectroscopic and mass spectrometric data.The results are summarized as follows:(1) Both the petroleum ether and ethyl acetate fractions exhibited inhibitory activities against Escherichia coli and Staphylococcus aureus,with the petroleum ether fraction outperforming the ethyl acetate fraction.(2)Both the petroleum ether and ethyl acetate fractions exhibited certain inhibitory effects on the proliferation of human lung cancer (A-549) cells and human cervical cancer (HeLa) cells.Moreover,the petroleum ether fraction showed significantly better inhibitory effects than the ethyl acetate fraction and a clear dose-dependent manner.(3)The median maximal inhibitory concentrations of the petroleum ether extract were 213.873 5 mg·L-1 against A-549 cells and 365.626 4 mg·L-1 against HeLa cells.(4)A total of nine monomeric compounds were separated from the S.ternatum extracts,including three previously reported compounds (luteolin,β-sitosterol,and daucosterol) and six compounds (hopane-22,29-diolmonoacetate,neoabietic acid,2',3'-dihydroxy propylpentadecanoate,docosanoic acid oxiranylmethyl ester,p-hydroxybenzoic acid,and benzerietridecanoic acid) separated for the first time from S.ternatum.This study has certain scientific significance for elucidating the components and biological activities of S.ternatum. |
| Key words: Sceptridium ternatum compound separation and identification antibacterial effects antitumor activity |
