| 摘要: |
| 本研究针对餐厨垃圾生物处理的关键问题——处理周期长且环境耐受性差，依据好氧生化处理思想，从餐厨垃圾环境中筛选好氧、耐高温、耐盐、耐油且高产蛋白酶的细菌菌株，为构建高效的“菌+酶”处理体系提供重要基础。通过富集驯化、平板分离和透明圈初筛（水解圈/菌落直径比），结合测定胞外蛋白酶活力，从餐厨垃圾样品中获得4株高产蛋白酶菌株；进一步选出最优菌株GXUN2927，鉴定为嗜麦芽寡养单胞菌（Stenotrophomonas maltophilia）。菌株GXUN2927的胞外蛋白酶活力为（108.69±2.731）U/mL，耐高温（45 °C）、耐盐（3% NaCl），兼具产脂肪酶能力（0.85 U/mL）。其蛋白酶粗酶液最适pH为7.5，最适宜温度为80 °C，在pH 5.0-9.5和温度30-90 °C下均保持稳定。Mn2?和β-巯基乙醇可显著提升酶催化效率（分别提高3.8倍和7.3倍）。菌株GXUN2927具备多重环境耐受性及高产高温稳定蛋白酶的特性，为餐厨垃圾高效降解与蛋白质资源化提供了优良菌种资源。 |
| 关键词: 餐厨垃圾 好氧处理 蛋白酶 嗜麦芽寡养单胞菌 酶学性质 |
| DOI： |
| 投稿时间：2025-05-15修订日期：2025-05-28 |
| 基金项目: |
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| Isolation of a Protease-Hyperproducing Stenotrophomonas maltophilia GXUN2927 from Food Waste and Characterization of Its Enzymatic Properties |
|
Chen Jia-tong, Liang Yu-rong, Chen Jie-qun, Ruan Ying-qi, Jiang Ming-guo, Zhang Zheng
|
| (Guangxi Minzu University) |
| Abstract: |
| This study addresses the challenges of prolonged processing cycles and poor environmental tolerance in current biotechnological treatments of food waste. Guided by aerobic biodegradation principles, we aimed to screen high-activity protease-producing strains from food waste to establish an efficient "bacteria-enzyme" synergistic system for enhanced resource recovery. Through enrichment cultivation, halo-forming screening (based on colony-to-hydrolysis zone ratio, D/d), and extracellular protease activity assays, four high-protease-producing strains were isolated. Further evaluation of thermotolerance (35–55°C), halotolerance (0–10% NaCl), and lipase production capacity identified the optimal strain, GXUN2927, which was taxonomically classified as Stenotrophomonas maltophilia. This strain demonstrated an extracellular protease activity of (108.69 ± 2.731) U/mL, exhibited tolerance to high temperature (45°C) and salinity (3% NaCl), and produced lipase (0.85 U/mL). The crude protease showed optimal activity at pH 7.5 and 80°C, with stability across a broad pH range (5.0–9.5) and temperature spectrum (30–90°C). Enzyme activity was significantly enhanced by Mn2?and β-mercaptoethanol (3.8-fold and 7.3-fold increases, respectively). Strain GXUN2927, characterized by multi-environmental resilience and high thermostable protease production, represents a promising microbial resource for efficient food waste degradation and protein valorization. |
| Key words: food waste aerobic treatment protease Stenotrophomonas maltophilia enzymatic properties |
