| 摘要: |
| 为解决西部半干旱煤矿区土壤养分贫瘠、微生物活性低、植物难存活等问题，以矿区原土和纯种植柠条锦鸡儿的盆栽为对照，研究丛枝菌根真菌(Arbuscular mycorrhiza fungi，AMF）单施，AMF与圆褐色固氮菌（Azotobacter chroococcum）、胶质芽孢杆菌（Bacillus mucilaginosus）、巨大芽孢杆菌（Bacillus megaterium）分别联合接种对柠条锦鸡儿（Caragana korshinskii）根系土壤理化性质、土壤酶活、球囊霉素相关土壤蛋白（GRSP）的差异以及对植株生长情况、生理特征、根系生长的影响。结果表明:与对照组相比，接种微生物处理均能显著提高柠条锦鸡儿根系土壤速效钾、速效磷、铵态氮和有机质含量，且能提高柠条锦鸡儿根系土壤脲酶、蔗糖酶和过氧化氢酶活性，促进柠条锦鸡儿生长。进一步研究显示:各处理组别均能提高柠条锦鸡儿的叶绿素a、类胡萝卜素以及可溶性蛋白含量，增强其抵抗不良环境能力。其中，AZBK（AMF+三种细菌）组别对土壤理化性质的改良作用以及促进植物生长和抗逆作用效果最明显，根系土壤有机质提高了95.1%，柠条锦鸡儿可溶性蛋白提高了225.0%。研究成果可为解决干旱半干旱煤矿区土壤肥力不足、植被恢复困难提供科学参考。 |
| 关键词: 丛枝菌根真菌 柠条锦鸡儿 半干旱煤矿区 促生作用 |
| DOI： |
| 投稿时间：2024-12-03修订日期：2025-01-19 |
| 基金项目:内蒙古自然科学基金(2023MS04010)；鄂尔多斯市科技重大专项(2021ZD社14-16)；国家自然科学(52264013)；内蒙古自治区直属高校基本科研业务费专项资金资助(2023CXPT004) |
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| The inoculation Effects of AMF Combined with Cunctional Bacteria on the Growth Promotion of Caragana korshinskii in Semi-arid Coal Mining Area |
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Jiangqinghong1,2, Yuruili1,2, Zhengchunli3, Whangzhe1,2, Zhangxinping4, Renjie4, Fengzhaochuo1,2
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| (1.Inner Mongolia University of Science &2.Technology;3.Shanghai Polytechnic University;4.Inner Mongolia Yitai Guanglian Coal Chemical Co., Ltd.) |
| Abstract: |
| To address the problems of poor soil nutrients, low microbial activity and the challenge of plant survival in the semi-arid coal mining area of western China, a study was conducted focusing on the single application of arbuscular mycorrhizal (AM) fungi in the original soil from the mining area and the potted plant of Caragana korshinskii as the control. The treatments involved the inoculation of AM fungi and the combination with Azotobacter chroococcum, Bacillus mucilaginosus, and B. megaterium, respectively. The differences in soil physical and chemical properties, soil enzyme activities, glomalin-related soil protein (GRSP), plant growth, physiological characteristics, and root growth were studied. The results showed that, compared with the control group, different microbial inoculation treatments could significantly increase the contents of available potassium, available phosphorus, ammonium nitrogen and organic matter in the rhizospheric soil of C. korshinskii. Additionally, these inoculations also increased the activities of urease, sucrase and catalase in the soil, thereby promoting the growth of C. korshinskii. Further studies showed that each treatment group led to an increase in the content of chlorophyll a, carotenoid, and soluble protein in C. korshinskii, thereby improving its ability to resist adverse environment. Among them, the AZBK group (AM fungi added with the three bacteria) demonstrated the most substantial improvements in the improvement of soil physical and chemical properties, as well as in plant growth and stress resistance. Specificially, the soil organic matter increased by 95.1 %, and the soluble protein in C. korshinskii increased by 225.0 %. The findings provide valuable scientific insights for addressing the shortage of soil fertility and aiding vegetation restoration in arid and semi-arid coal mining areas. |
| Key words: Arbuscular mycorrhizal fungi Caragana korshinskii Semi-arid coal mining area Growth-promoting effect |
