| 引用本文： |
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王尚涛,朱成豪,江媛,张远帆,王彦珺,张淑睿,孙志蓉.光照强度对美花石斛试管苗生长及光合特性的影响[J].广西科学,2023,30(6):1156-1162. [点击复制]
- WANG Shangtao,ZHU Chenghao,JIANG Yuan,ZHANG Yuanfan,WANG Yanjun,ZHANG Shurui,SUN Zhirong.Effects of Light Intensity on the Growth and Photosynthetic Characteristics of Dendrobium loddigesii in vitro[J].Guangxi Sciences,2023,30(6):1156-1162. [点击复制]
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| 摘要: |
| 为探究光照强度对美花石斛（Dendrobium loddigesii）试管苗生长及光合特性的影响，研究对不同光照强度处理2个月和4个月的美花石斛试管苗的生长情况、多糖含量、光合特性和荧光特性进行测定和分析。结果显示，随着光照强度的增大，处理2个月和4个月的美花石斛试管苗的生长指标和多糖含量均显著增大；在光照处理2个月时，36 μmol·m-2·s-1的光照强度有助于试管苗光系统Ⅱ （PSⅡ）最大光化学效率的提高和热耗散能力的增强，而54 μmol·m-2·s-1的光照强度有助于试管苗碳循环系统的完善；在光照处理4个月时，54 μmol·m-2·s-1光照强度有利于试管苗光反应系统的完善和热耗散能力的增强。研究结果表明，美花石斛试管苗在不同发育时期对光照强度的要求不同，培育过程中可根据试管苗的生长节律进行光照强度的调节。 |
| 关键词: 美花石斛 试管苗 生长发育 光合特性 荧光特性 |
| DOI：10.13656/j.cnki.gxkx.20240125.014 |
| 投稿时间：2023-03-16修订日期：2023-06-03 |
| 基金项目:国家现代农业产业技术体系项目（CARS-21）和广西林业局科技项目（2023LYKJ03）资助。 |
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| Effects of Light Intensity on the Growth and Photosynthetic Characteristics of Dendrobium loddigesii in vitro |
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WANG Shangtao, ZHU Chenghao, JIANG Yuan, ZHANG Yuanfan, WANG Yanjun, ZHANG Shurui, SUN Zhirong
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| (School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, China) |
| Abstract: |
| To study the effects of light intensity on the growth and photosynthetic characteristics of the test-tube seedlings of Dendrobium loddigesii, the growth indexes, polysaccharide content, photosynthetic and fluorescent characteristics of the test-tube seedlings of D.loddigesii treated with different light intensity for 2 months and 4 months were determined and analyzed. The results showed that the growth indexes and polysaccharide content of the test-tube seedlings of D.loddigesii at 2 months and 4 months were significantly increased with the light intensity. After 2 months of light treatment, 36 μmol·m-2·s-1 light intensity was helpful to improve the quantum efficiency and heat dissipation capacity of PSⅡ seedlings, while 54 μmol·m-2·s-1 light intensity was helpful to improve the carbon cycling system of the test-tube seedlings of D.loddigesii.After 4 months of light treatment, 54 μmol·m-2·s-1 light intensity was beneficial for the improvement of light system and the enhancement of heat dissipation ability.It is suggested that the light intensity requirements of the test-tube seedlings of D.loddigesii were different at different developmental stages, and the light intensity could be adjusted according to the growth rhythm of test-tube seedlings. |
| Key words: Dendrobium loddigesii plantlets in vitro growth and development photosynthetic characteristics fluorescence characteristic |
