| 引用本文： |
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邓丽丽,唐健民,邹蓉,杨一山,陈泰国,韦霄.广西崇左叉叶苏铁野生和迁地保护种群的遗传多样性比较分析[J].广西科学,2023,30(6):1101-1109. [点击复制]
- DENG Lili,TANG Jianmin,ZOU Rong,YANG Yishan,CHEN Taiguo,WEI Xiao.Comparative Analysis of the Genetic Diversity between Wild Population and Ex Situ Conservation Population of Cycas bifida (Dyer) K.D.Hill in Chongzuo City of Guangxi[J].Guangxi Sciences,2023,30(6):1101-1109. [点击复制]
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| 摘要: |
| 叉叶苏铁[Cycas bifida （Dyer） K.D.Hill]为国家一级重点保护野生植物，了解广西崇左叉叶苏铁野生种群和迁地保护种群的遗传多样性和遗传结构，可为其种质资源的保护及管理提供指导。本研究利用筛选得到的6对稳定且多态性良好的Simple Sequence Repeat （SSR）引物，对位于崇左的4个叉叶苏铁野生种群和迁至桂林的1个叉叶苏铁迁地保护种群共109个个体的遗传多样性和遗传结构进行分析。结果表明： 4个野生种群的观测等位基因数平均值为2.875，有效等位基因数平均值为1.796，Shannon信息指数平均值为0.637，观测杂合度平均值为0.363，期望杂合度平均值为0.373，说明广西崇左叉叶苏铁遗传多样性水平较低。迁地保护种群的观测等位基因数、有效等位基因数、Shannon信息指数、观测杂合度和期望杂合度分别为3.000、1.865、0.686、0.323和0.391，说明迁地保护能提高叉叶苏铁遗传多样性。遗传结构中群体间遗传呈中度分化（0.134），种群间遗传变异占25%，种群内遗传变异占75%，遗传变异主要来源于种群内。非加权组平均法（Unweighted Pair-Group Method with Arithmetic means，UPGMA）、主坐标分析（Principal Co-ordinates Analysis，PCoA）和Structure分析表明109份样品可分为2类，迁地保护种群ZWS和野外种群PR为一类，其余3个种群为一类。野生种群中MALL的群体遗传多样性最高（Shannon信息指数为0.711，期望杂合度为0.394），应将该种群作为重点保护单元进行保护。迁地保护种群ZWS的Shannon信息指数和期望杂合度均高于野生种群平均值，但Shannon信息指数略低于野生种群MALL，期望杂合度略低于野生种群MALL和YCLL。建议继续加强野生种群MALL和YCLL种群资源的引种工作，以便更有效地保护叉叶苏铁的遗传资源。 |
| 关键词: 叉叶苏铁 野生种群 迁地保护种群 SSR 遗传多样性 遗传结构 |
| DOI：10.13656/j.cnki.gxkx.20240125.008 |
| 投稿时间：2023-09-08修订日期：2023-11-15 |
| 基金项目:国家重点研发计划项目（2022YFF1300703），中国科学院“西部之光”计划（2022），广西林业科技推广示范项目（〔2022〕GT23）和广西植物功能性植物化学物质研究与可持续利用重点实验室自主项目（ZRJJ2022-2）资助。 |
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| Comparative Analysis of the Genetic Diversity between Wild Population and Ex Situ Conservation Population of Cycas bifida (Dyer) K.D.Hill in Chongzuo City of Guangxi |
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DENG Lili1, TANG Jianmin1, ZOU Rong1, YANG Yishan1, CHEN Taiguo1,2, WEI Xiao1
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| (1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China;2.College of Pharmacy, Guilin Medical University, Guilin, Guangxi, 541199, China) |
| Abstract: |
| Cycas bifida (Dyer) K.D.Hill is a national level Ⅰ protection of wild plants. The genetic diversity and structure of wild population and ex situ conservation population of C.bifida (Dyer) K.D.Hill in Chongzuo City of Guangxi will provide a better guidance for the formulation of resource conservation and management strategies. In this study, six pairs of SSR primers with good stability and polymorphism were selected to analyze the genetic diversity and genetic structure of 109 C.bifida (Dyer) K.D.Hill individuals from 4 wild populations in Chongzuo and 1 ex situ conservation population in Guilin. The results showed that:The average number of observed allele, the average number of effective allele, the average Shannon's information index, the average observed heterozygosity and the average expected heterozygosity of 4 wild populations were 2.875, 1.769, 0.637, 0.363 and 0.373 respectively. The level of genetic diversity of C.bifida (Dyer) K.D.Hill in Chongzuo was low. The number of observed allele, the number of effective allele, the Shannon's information index, the observed heterozygosity and the expected heterozygosity of ex situ conservation population were 3.000, 1.865, 0.686, 0.323 and 0.391 respectively, indicating ex situ conservation can improve the genetic diversity of C.bifida (Dyer) K.D.Hill. The genetic differentiation among populations was moderate (0.134) which is mainly contributed by intra-population variation (accounts for 75%). Ex situ conservation population ZWS and wild population PR were classified in one group via UPGMA, PCoA and structure analyses, whereas the rest samples were in the other group. The genetic diversity of MALL was the highest among the wild populations, which should be protected as a key protection unit. The Shannon's information index and expected heterozygosity of ex situ conservation population ZWS were higher than the average value of wild populations, but Shannon's information index was slightly lower than that of wild population MALL and expected heterozygosity was slightly lower than that of wild population MALL and wild population YCLL. It is suggested that the introduction of wild population MALL and wild population YCLL population resources should be strengthened in order to protect the genetic resources of C.bifida (Dyer) K.D.Hill more effectively. |
| Key words: Cycas bifida (Dyer) K.D.Hill wild population ex situ conservation population SSR genetic diversity genetic structure |
