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何佳利,甘雨满,白猛,高程海,张文飞,王锐萍.蜡样芽孢杆菌BWL-001代谢产物及抗弧菌活性研究[J].广西科学,2023,30(4):713-718. [点击复制]
- HE Jiali,GAN Yuman,BAI Meng,GAO Chenghai,ZHANG Wenfei,WANG Ruiping.Study on Metabolites and Anti-Vibrio Activity of Bacillus cereus BWL-001[J].Guangxi Sciences,2023,30(4):713-718. [点击复制]
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| 蜡样芽孢杆菌BWL-001代谢产物及抗弧菌活性研究 |
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何佳利1,2,3, 甘雨满3,4, 白猛3,4, 高程海3,4, 张文飞1,2, 王锐萍1,2
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| (1.海南师范大学生命科学学院, 海南海口 571158;2.海南师范大学, 热带岛屿生态学教育部重点实验室, 海南海口 571158;3.广西中医药大学海洋药物研究院, 广西南宁 530200;4.广西中医药大学, 广西海洋药物重点实验室, 广西南宁 530200) |
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| 摘要: |
| 本研究旨在从1株海南霸王岭热带雨林土壤来源的蜡样芽孢杆菌(Bacillus cereus) BWL-001中挖掘抗海洋弧菌(Vibrio)的活性代谢产物，采用多种现代色谱分离方法对菌株提取物进行分离纯化，通过核磁共振波谱解析确定单体化合物结构，并结合96孔板微量稀释法进行抗弧菌活性测试。结果显示，从该菌株LB培养基发酵产物中分离鉴定出10个单体化合物，包括4个二酮哌嗪类化合物(1-4)、3个吲哚生物碱衍生物(6-8)以及3个其他类化合物(5、9和10)，分别命名为3-异丁基吡咯并哌嗪-2,5-二酮(1)、cyclo-(Pro-lle) (2)、maculosin (3)、trans-cyclo-(Pro-Phe) (4)、anthranilic acid (5)、indole-3-meth-ylethanoate (6)、indole-3-acetic acid (7),nb-acetyltryptamine (8)、thymine (9)、acetamide (10)。其中，化合物6-8对副溶血弧菌(Vibrio parahaemolyticus)有弱的抑制活性，其最小抑菌浓度(Minimal Inhibit Concentration，MIC)值均为12.5 μg/mL。本研究丰富了海南霸王岭热带雨林土壤来源的蜡样芽孢杆菌代谢产物多样性，并为抗弧菌活性先导化合物的发现提供理论基础。 |
| 关键词: 霸王岭热带雨林 土壤 蜡样芽孢杆菌 代谢产物 抗弧菌活性 |
| DOI：10.13656/j.cnki.gxkx.20230928.010 |
| 投稿时间：2023-03-29修订日期：2023-05-05 |
| 基金项目:国家自然科学基金地区项目(31960012)，海南省自然科学基金高层次人才项目(321RC545)和海南省重大科技计划项目(ZDKJ2021035)资助。 |
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| Study on Metabolites and Anti-Vibrio Activity of Bacillus cereus BWL-001 |
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HE Jiali1,2,3, GAN Yuman3,4, BAI Meng3,4, GAO Chenghai3,4, ZHANG Wenfei1,2, WANG Ruiping1,2
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| (1.College of Life Sciences, Hainan Normal University, Haikou, Hainan, 571158, China;2.Key Laboratory of Tropical Islands Ecology, Ministry of Education, Hainan Normal University, Haikou, Hainan, 571158, China;3.Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China;4.Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China) |
| Abstract: |
| The aim of this study was to explore the active metabolites against marine Vibrio from a strain of Bacillus cereus BWL-001 isolated from the soil of Bawangling tropical rainforest in Hainan.A variety of modern chromatographic separation methods were used to isolate and purify the extracts of the strain.The structure of the monomer compound was determined by nuclear magnetic resonance spectroscopy,and the anti-Vibrio activity was tested by 96-well plate micro-dilution method.The results showed that 10 monomer compounds were isolated and identified from LB culture medium of the strain,including four diketopiperazines (1-4),three indole alkaloid derivatives (6-8),and three other compounds (5,9 and 10).Named 3-isobutylpyrrolopiperazine-2,5-dione (1),cyclo-(Pro-lle) (2),maculosin (3),trans-cyclo-(Pro-Phe) (4),anthranilic acid (5),indole-3-meth-ylethanoate (6),indole-3-acetic acid (7),nb-acetyltryptamine (8),thymine (9),acetamide (10).Among them,compounds 6-8 had weak inhibitory activity against Vibrio parahaemolyticus with Minimal Inhibit Concentration (MIC) values of 12.5 μg/mL.This study enriched the diversity of metabolites of B.cereus derived from the soil of Bawangling tropical rainforest in Hainan,and provided a theoretical basis for the discovery of lead compounds with anti-Vibrio activity. |
| Key words: Bawangling tropical rain forest soil Bacillus cereus metabolites anti-Vibrio activity |
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