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朱鹏锦,宋奇琦,谭秦亮,程琴,李佳慧,庞新华,周全光,吕平,欧克纬,卢业飞,农泽梅.3个甘蔗品种响应低温胁迫的转录组分析[J].广西科学,2023,30(2):267-276. [点击复制]
- ZHU Pengjin,SONG Qiqi,TAN Qinliang,CHENG Qin,LI Jiahui,PANG Xinhua,ZHOU Quanguang,Lü Ping,OU Kewei,LU Yefei,NONG Zemei.Transcriptome Analysis of Three Sugarcane Varieties in Response to Low Temperature Stress[J].Guangxi Sciences,2023,30(2):267-276. [点击复制]
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| 3个甘蔗品种响应低温胁迫的转录组分析 |
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朱鹏锦, 宋奇琦, 谭秦亮, 程琴, 李佳慧, 庞新华, 周全光, 吕平, 欧克纬, 卢业飞, 农泽梅
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| (广西壮族自治区亚热带作物研究所, 广西南宁 530001) |
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| 摘要: |
| 为挖掘甘蔗(Saccharum officinarum L.)响应低温的特异基因，揭示甘蔗响应低温的分子调控机理，以3个抗寒能力不同的甘蔗品种作为试验材料，利用转录组测序技术分析不同抗寒能力的甘蔗品种在低温胁迫(4 ℃)下的基因表达差异。结果表明，利用权重基因共表达网络分析(Weighted Gene Co-expression Network Analysis，WGCNA)鉴定出13个基因共表达模块，通过相关性分析筛选出blue和yellow模块作为甘蔗抗寒机理研究的目标模块。基于blue和yellow模块筛选出可能与甘蔗抗寒能力密切相关的13个响应低温胁迫的基因，为后续选育抗寒性强的优良甘蔗新品种提供重要参考。 |
| 关键词: 甘蔗 低温 转录组 权重基因共表达网络分析 |
| DOI：10.13656/j.cnki.gxkx.20230118.001 |
| 投稿时间：2022-06-20修订日期：2022-09-30 |
| 基金项目:广西自然科学基金项目(2017GXNSFBA198054)，广西科技重大专项(桂科AA17202042-5)和广西农业科学院科技发展基金项目(桂农科2022JM90,桂农科2021YT151)资助。 |
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| Transcriptome Analysis of Three Sugarcane Varieties in Response to Low Temperature Stress |
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ZHU Pengjin, SONG Qiqi, TAN Qinliang, CHENG Qin, LI Jiahui, PANG Xinhua, ZHOU Quanguang, Lü Ping, OU Kewei, LU Yefei, NONG Zemei
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| (Guangxi Subtropical Crops Research Institute, Nanning, Guangxi, 530001, China) |
| Abstract: |
| In order to explore the specific genes of sugarcane (Saccharum officinarum L.) in response to low temperature and reveal the molecular regulation mechanism of sugarcane in response to low temperature,three sugarcane varieties with different cold resistance were used as experimental materials,and the gene expression differences of sugarcane varieties with different cold resistance under low temperature stress (4 ℃) were analyzed by transcriptome sequencing technology.The research results showed that 13 gene co-expression modules were identified by Weighted Gene Co-expression Network Analysis (WGCNA),and blue and yellow modules were selected as the target modules for the study of sugarcane cold resistance mechanism by correlation analysis.Based on blue and yellow modules,13 genes responding to low temperature stress that may be closely related to the cold resistance of sugarcane were screened,which provided an important reference for the subsequent selecting and breeding of new sugarcane varieties with strong cold resistance. |
| Key words: sugarcane low temperature transcriptome Weighted Gene Co-expression Network Analysis |
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