| 引用本文： |
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罗舒心,刘仲钰,梁明坤,钟振国,钟静.玛卡调控5-HT、DA对小鼠自闭症样行为及神经环路的影响[J].广西科学,2022,29(5):959-970. [点击复制]
- LUO Shuxin,LIU Zhongyu,LIANG Mingkun,ZHONG Zhenguo,ZHONG Jing.Effects of Maca by Regulating 5-HT and DA on Autism-like Behavior and Neural Circuit in Mice[J].Guangxi Sciences,2022,29(5):959-970. [点击复制]
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| 摘要: |
| 通过研究玛卡调控5-羟色胺(5-hydroxytryptamine，5-HT)、多巴胺(Dopamine，DA)对小鼠自闭症样行为及相关神经环路的影响，初步探讨玛卡治疗自闭症的疗效及其作用机制。在整个实验中将ICR小鼠及C57/BL6小鼠分别随机设空白对照组及玛卡低、中、高剂量组，采用灌胃给药的方式，并通过高架十字迷宫实验、旷场实验及三腔社交实验，观察玛卡给药后两种不同种系小鼠行为学的变化；采用酶联免疫试剂盒检测小鼠血浆5-HT及DA含量；通过c-fos蛋白检测相关脑区的神经元活性。结果显示，与空白对照组比较，玛卡提高了ICR小鼠在高架十字迷宫实验中进入开放臂的时间、次数及指数(P＜0.05或P＜0.01)，减少了其在旷场实验中的总移动距离(P＜0.05或P＜0.01)，提高了其在三腔社交实验中的社会性指数、社会新颖性指数与社会记忆指数(P＜0.05)，并提高了其血浆5-HT及DA含量(P＜0.05或P＜0.01或P＜0.001)；c-fos检测结果显示，VTA、VP、NAcc、mPOA及amygdala阳性细胞数目明显增多(P＜0.05或P＜0.01或P＜0.001)。本研究结果提示，玛卡对ICR小鼠自闭症样行为有明显的改善作用，且该作用可能是通过调节5-HT及DA的水平并影响相关的神经环路实现的。 |
| 关键词: 玛卡 自闭症 社会行为 5-羟色胺 多巴胺 社会脑 机制 |
| DOI：10.13656/j.cnki.gxkx.20221116.017 |
| 投稿时间：2021-10-12修订日期：2021-12-03 |
| 基金项目:国家自然科学基金项目(81960807)，广西自然科学基金青年科学基金项目(2018GXNSFBA138008)，广西高校引进海外高层次人才“百人计划”，2017年广西高校中青年教师基础能力提升项目(2017KY0283)，广西自然科学基金面上项目(2018GXNSFAA281225)和广西中医药大学高层次人才队伍建设三年行动计划“桂派杏林青年英才”项目资助。 |
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| Effects of Maca by Regulating 5-HT and DA on Autism-like Behavior and Neural Circuit in Mice |
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LUO Shuxin1, LIU Zhongyu1, LIANG Mingkun2, ZHONG Zhenguo1, ZHONG Jing3,4
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| (1.Scientific Experimental Center of Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China;2.Neurology Department, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi, 530011, China;3.School of Basic Medicine of Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China;4.Postdoctoral Workstation, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi, 530023, China) |
| Abstract: |
| By studying the effects of Maca regulating 5-hydroxytryptamine (5-HT) and dopamine (DA) on autism-like behavior and related neural circuits in mice,the efficacy and mechanism of Maca in the treatment of autism were preliminarily discussed.In the whole experiment,ICR mice and C57/BL6 mice were randomly divided into blank control group and Maca low-,medium- and high-dose groups.The drug was administered by intragastric gavage,and the elevated cross labyrinth test,open field test and three-chamber social test were carried out to observe the behavioral changes of two different strains of mice after Maca administration.The contents of 5-HT and DA in plasma of mice were detected by enzyme-linked immunosorbent assay kit.The activity of neurons in related brain regions was detected by c-fos protein.The results showed compare with the blank control group,Maca increased the time,times and index of entering the open arm in the elevated cross labyrinth test of ICR mice (P＜0.05 or P＜0.01),decreased the total distance in the open field test (P＜0.05 or P＜0.01),and increased the social index,social novelty index and social memory index in the three chambers test (P＜0.05).The contents of 5-HT and DA in plasma were increased (P＜0.05 or P＜0.01 or P＜0.001).The results of c-fos detection showed that the number of positive cells in VTA,VP,NAcc,mPOA and amygdala increased significantly (P＜0.05 or P＜0.01 or P＜0.001).The results of this study suggest that Maca has a significant improvement effect on autism-like behavior in ICR mice,and this effect may be achieved by regulating the levels of 5-HT and DA and affecting the related neural circuits. |
| Key words: Maca autism social behavior 5-HT DA social brain mechanism |
