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薛欣怡,张翼,廖清楠,冯昀铠,胡雪琼,刘亚月.添加前体对2株真菌次级代谢产物及其生物活性的影响[J].广西科学,2022,29(5):854-862. [点击复制]
- XUE Xinyi,ZHANG Yi,LIAO Qingnan,FENG Yunkai,HU Xueqiong,LIU Yayue.Effects of Adding Precursors on Secondary Metabolites and Their Activities of Two Fungal Strains[J].Guangxi Sciences,2022,29(5):854-862. [点击复制]
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| 添加前体对2株真菌次级代谢产物及其生物活性的影响 |
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薛欣怡1, 张翼1,2,3, 廖清楠1, 冯昀铠1, 胡雪琼1, 刘亚月1,2,3
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| (1.广东海洋大学食品科技学院, 广东省水产品加工与安全重点实验室, 广东省海洋生物制品工程实验室, 广东省海洋食品工程技术研究中心, 水产品深加工广东普通高等学校重点实验室, 湛江市脑健康海洋药物与营养品重点实验室, 广东海洋大学海洋药物研究所, 广东湛江 524088;2.广东海洋大学深圳研究院海洋医药研发中心, 广东深圳 518120;3.大连工业大学, 海洋食品精深加工关键技术省部共建协同创新中心, 辽宁大连 116034) |
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| 摘要: |
| 为探究添加前体物质对红树植物杨叶肖槿(Thespesia populnea)来源内生真菌Talaromyces sp.YX-001和Penicillium sp.YX-002次级代谢产物及其生物活性的影响，选取马铃薯葡萄糖液体(Potato Dextrose Broth，PDB)培养基和察氏(Czapek's)培养基，分别在其中添加2种前体物质(甲戊二羟酸和酪氨酸)，采用12孔板对菌株进行微量发酵培养。通过分析菌株次级代谢产物的产量、HPLC指纹图谱、乙酰胆碱酯酶(AChE)抑制活性、1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除活性和卤虫致死活性，探究添加前体物质前后菌株次级代谢产物的化学多样性和生物活性的变化情况。结果表明：添加2种前体物质均可使菌株次级代谢产物的产量和化学多样性增加，但生物活性的变化有所不同。酸性环境可能会促进菌株中具有较好DPPH自由基清除活性的次级代谢产物的合成，但其具体机制还有待进一步研究。本研究为深入研究杨叶肖槿内生真菌Talaromyces sp.YX-001和Penicillium sp.YX-002的次级代谢产物奠定了基础。 |
| 关键词: 前体物质 杨叶肖槿 Talaromyces sp. Penicillium sp. 次级代谢产物 |
| DOI：10.13656/j.cnki.gxkx.20221116.005 |
| 投稿时间：2022-05-18修订日期：2022-06-27 |
| 基金项目:国家自然科学基金项目(21807015)，广东海洋大学博士启动项目(R18008)，湛江市科技发展专项资金竞争性分配项目(2020A01030)，广东省扬帆计划引进紧缺拔尖人才项目(201433009)，深圳市科创委基础研究面上项目(JCYJ20190813105005619)，深圳市大鹏新区科技研发项目(KJYF202001-07)，广东省普通高校重点领域专项“生物医药与健康”(2021ZDZX2064)，广东省基础与应用基础研究基金项目(2021A50114)和湛江市海洋经济创新发展示范市建设项目(XM-202008-01B1)资助。 |
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| Effects of Adding Precursors on Secondary Metabolites and Their Activities of Two Fungal Strains |
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XUE Xinyi1, ZHANG Yi1,2,3, LIAO Qingnan1, FENG Yunkai1, HU Xueqiong1, LIU Yayue1,2,3
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| (1.College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China;2.Center for Marine Drugs Research & Development, Shenzhen Institute of Guangdong Ocean University, Shenzhen, Guangdong, 518120, China;3.Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, Liaoning, 116034, China) |
| Abstract: |
| In order to explore the effect of adding precursor substances on the secondary metabolites and their bioactivity of endophytic fungi Talaromyces sp.YX-001 and Penicillium sp.YX-002 from the mangrove plant Thespesia populnea,Potato Dextrose Broth (PDB) and Czapek's medium were selected,and two kinds of precursors (mevalondihydroxy acid and tyrosine) were added to them respectively.The strain was cultured by micro-fermentation in 12-well plates.By analyzing the yield of secondary metabolites,HPLC fingerprint,acetylcholinesterase (AChE) inhibitory activity，1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical scavenging activity and artemia lethal activity,the chemical diversity and biological activity of secondary metabolites before and after the addition of precursor substances were investigated.The results showed that the addition of two precursors could increase the yield and chemical diversity of secondary metabolites,but the changes in biological activity were different.The acidic environment might promote the synthesis of secondary metabolites with good DPPH free radical scavenging activity in the strain,but the specific mechanism remains to be further studied.The study lays a foundation for further research on the secondary metabolites of the endophytic fungi Talaromyces sp.YX-001 and Penicillium sp.YX-002. |
| Key words: precursor substances Thespesia populnea Talaromyces sp. Penicillium sp. secondary metabolites |
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