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谭玉莹,曹广萍,黄炯丽,李秋庭,唐振洲,刘永宏,高程海,白猛.红树老鼠簕来源内生青霉Penicillium sp.GXIMD 03101的次级代谢产物研究[J].广西科学,2022,29(5):839-845. [点击复制]
- TAN Yuying,CAO Guangping,HUANG Jiongli,LI Qiuting,TANG Zhenzhou,LIU Yonghong,GAO Chenghai,BAI Meng.Studies on Secondary Metabolites of Endophytic Fungus Penicillium sp.GXIMD 03101 from Mangrove Acanthus ilicifolius L.[J].Guangxi Sciences,2022,29(5):839-845. [点击复制]
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| 红树老鼠簕来源内生青霉Penicillium sp.GXIMD 03101的次级代谢产物研究 |
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谭玉莹1, 曹广萍2, 黄炯丽1, 李秋庭1, 唐振洲2, 刘永宏2, 高程海2, 白猛2
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| (1.广西大学轻工与食品工程学院, 广西南宁 530004;2.广西中医药大学海洋药物研究院, 广西南宁 530200) |
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| 摘要: |
| 为了研究红树老鼠簕Acanthus ilicifolius L.来源内生青霉Penicillium sp.GXIMD 03101的次级代谢产物，采用硅胶柱层析、凝胶柱层析及半制备高效液相色谱对大米发酵产物进行分离纯化，利用核磁共振方法鉴定化合物的结构，并对化合物1-4进行抗胰腺癌活性筛选。结果显示，从内生青霉Penicillium sp.GXIMD 03101中分离鉴定出13个化合物，分别为 (+)-rugulosin A (1)、(+)-rugulosin B (2)、1,1',3,3',5,5'-hexahydroxy-7,7'-dimethyl[2,2'-bianthracene]-9,9',10,10'-tetraone (3)、questin (4)、emodin (5)、questinol (6)、uridine (7)、2'-deoxythymidine (8)、5'-O-acetyl uridine (9)、ergosta-5,7,22-triene-3β-ol (10)、(3R)-6-hydroxymellein (11)、6-hydroxy-8-methoxy-3,4-dimethylisocoumarin (12) 和indole-3-acetic acid (13)。其中，化合物 1 对胰腺癌细胞表现出较好的细胞毒活性，其浓度为10 μmol/L时对胰腺癌细胞的抑制率为75.6%。以上结果为进一步研究开发红树内生青霉来源的天然抗胰腺癌药物提供了物质基础。 |
| 关键词: 红树老鼠簕 内生真菌 Penicillium sp. 次级代谢产物 抗胰腺癌活性 |
| DOI：10.13656/j.cnki.gxkx.20221116.003 |
| 投稿时间：2021-12-27修订日期：2022-02-10 |
| 基金项目:广西自然科学基金项目(2021GXNSFBA220072，2020GXNSFGA297002),广西高校中青年教师科研基础能力提升项目(2021KY0318)，广西中医药大学2020 年博士科研启动基金项目(2020BS024 )和广西中医药大学八桂学者项目(05019055)资助。 |
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| Studies on Secondary Metabolites of Endophytic Fungus Penicillium sp.GXIMD 03101 from Mangrove Acanthus ilicifolius L. |
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TAN Yuying1, CAO Guangping2, HUANG Jiongli1, LI Qiuting1, TANG Zhenzhou2, LIU Yonghong2, GAO Chenghai2, BAI Meng2
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| (1.College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi, 530004, China;2.Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China) |
| Abstract: |
| In order to investigate the secondary metabolites of an endophytic fungus Penicillium sp.GXIMD 03101 from mangrove Acanthus ilicifolius L.,the rice fermentation products were isolated and purified by silica gel column chromatography,gel column chromatography and semi-preparative High Performance Liquid Chromatography (HPLC).The structures of the compounds were identified by Nuclear Magnetic Resonance (NMR).And compounds 1-4 were screened for anti-pancreatic cancer activity.The results showed that 13 compounds were isolated and identified from Penicillium sp.GXIMD 03101,which were (+)-rugulosin A (1),(+)-rugulosin B (2),1,1',3,3',5,5'-hexahydroxy-7,7'-dimethyl[2,2'-bianthracene]-9,9',10,10'-tetraone (3),questin (4),emodin (5),questinol (6),uridine (7),2'-deoxythymidine (8),5'-O-acetyl uridine (9),ergosta-5,7,22-triene-3-β-ol (10),(3R)-6-hydroxymellein (11),6-hydroxy-8-methoxy-3,4-dimethylisocoumarin (12) and indole-3-acetic acid (13).Among them,compound 1 showed good cytotoxic activity against pancreatic cancer cells,and the inhibition rate was 75.6% at the concentration of 10 μmol/L.The above results provide a material basis for further research and development of natural anti-pancreatic cancer drugs derived from mangrove endophytic Penicillium. |
| Key words: Acanthus ilicifolius L. endophytic fungus Penicillium sp. secondary metabolites anti-pancreatic cancer activity |
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