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李魁鹏,陈仕昌,程琳,贺锦锋,唐红亮,谭文婧,余代渊,王斌,莫宗恒,黄开勇.基于SSR标记构建广西杉木核心种质[J].广西科学,2021,28(5):511-519. [点击复制]
- LI Kuipeng,CHEN Shichang,CHENG Lin,HE Jinfeng,TANG Hongliang,TAN Wenjing,YU Daiyuan,WANG Bin,MO Zongheng,HUANG Kaiyong.Construction of Core Germplasm of Cunninghamia lanceolata in Guangxi Based on SSR Markers[J].Guangxi Sciences,2021,28(5):511-519. [点击复制]
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| 基于SSR标记构建广西杉木核心种质 |
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李魁鹏1, 陈仕昌1, 程琳1, 贺锦锋2, 唐红亮3, 谭文婧2, 余代渊2, 王斌3, 莫宗恒3, 黄开勇1
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| (1.广西壮族自治区林业科学研究院, 广西优良用材林资源培育重点实验室, 广西南宁 530002;2.融水苗族自治县国营贝江河林场, 广西柳州 545300;3.融安县西山林场, 广西柳州 545400) |
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| 摘要: |
| 为深入发掘和筛选广西杉木Cunninghamia lanceolata种质资源，本研究采用SSR标记技术检测国家级杉木良种基地864份杉木种质材料的遗传多样性，对比分析不同构建方法的差异，并采用t检验和主坐标分析法验证核心种质的有效性和代表性。结果表明：（1）22对SSR引物共检测到195个等位基因，平均等位基因（Na）为8.86，平均有效等位基因（Ne）为3.13，平均Shannon's信息指数（I）为1.31，平均无效等位基因频率（Fna）为0.03，平均多态信息含量（PIC）为0.60。（2）相较其他构建方法，采用M策略的Core Finder软件是构建广西杉木核心种质的有效方法，筛选出的80份核心种质具较好的代表性。 |
| 关键词: 杉木 SSR标记 M策略 核心种质 广西 |
| DOI：10.13656/j.cnki.gxkx.20211203.003 |
| 投稿时间：2021-03-28 |
| 基金项目:广西创新驱动发展专项（桂科AA17204087-2），广西自然科学基金项目（2018GXNSFAA294083），广西主要用材林资源高效培育与利用人才小高地专项（桂财社函[2018]112）和广西林业科技项目（桂林科字[2016]第36号）资助。 |
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| Construction of Core Germplasm of Cunninghamia lanceolata in Guangxi Based on SSR Markers |
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LI Kuipeng1, CHEN Shichang1, CHENG Lin1, HE Jinfeng2, TANG Hongliang3, TAN Wenjing2, YU Daiyuan2, WANG Bin3, MO Zongheng3, HUANG Kaiyong1
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| (1.Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Zhuang Autonomous Region Forestry Research Institute, Nanning, Guangxi, 530002, China;2.State-owned Beijianghe Forest Farm, Rongshui Miao Autonomous County, Liuzhou, Guangxi, 545300, China;3.Rong'an State-owned Xishan Forest Farm, Liuzhou, Guangxi, 545400, China) |
| Abstract: |
| In order to explore and screen the Cunninghamia lanceolata germplasm resources in Guangxi, the genetic diversity of 864 C.lanceolata germplasm materials in national C.lanceolata improved seed base was detected by SSR marker technology, the differences of different construction methods were compared and analyzed, and the effectiveness and representativeness of core germplasm were verified by t-test and principal coordinate analysis.The results showed that:(1) a total of 195 alleles were detected by 22 pairs of SSR primers, the average allele (Na) was 8.86,the average effective allele (Ne) was 3.13,the average Shannon's information index (I) was 1.31,the average invalid allele frequency (Fna) was 0.03,and the average polymorphism information content (PIC) was 0.60.(2) Compared with other construction methods, the Core Finder software using M strategy was an effective method to construct the core germplasm of C.lanceolata in Guangxi.The 80 core germplasm screened out had good representativeness. |
| Key words: Cunninghamia lanceolata SSR marker M strategy core germplasm Guangxi |
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