| 引用本文： |
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郭勇秀,蒋林,农毅清,李芳婵,随家宁,魏博伟.一测多评法测定壮药三加皮中5个酚酸类成分的含量[J].广西科学,2021,28(4):416-422. [点击复制]
- GUO Yongxiu,JIANG Lin,NONG Yiqing,LI Fangchan,SUI Jianing,WEI Bowei.Determination of 5 Phenolic Acid Components in Zhuang Medicine Acanthopanax trifoliatus (L.)Merr. Based on Quantitative Analysis of Multi-component with Single-marker[J].Guangxi Sciences,2021,28(4):416-422. [点击复制]
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| 摘要: |
| 为建立一种可同时测定三加皮中5种酚酸类成分（新绿原酸、绿原酸、异绿原酸B、异绿原酸A、异绿原酸C）含量的一测多评法（QAMS），以绿原酸为内参物，采用色谱柱phenomenex C18（4.6 mm×250 mm，5 μL），以乙腈（C）-0.2%磷酸（A）为流动相进行梯度洗脱，流速1.0 mL/min，柱温30℃，检测波长325 nm。建立新绿原酸、异绿原酸B、异绿原酸A、异绿原酸C与内参物绿原酸的相对校正因子，计算待测成分的含量，与外标法（ESM）测定的结果比较，评价QAMS的可行性与准确性。结果显示，所建立的三加皮中新绿原酸、绿原酸、异绿原酸B、异绿原酸A、异绿原酸C的相对校正因子重现性良好；10个产地的三加皮中5种成分QAMS计算值与ESM实测值比较无显著差异。本研究建立的一测多评法（QAMS）可应用于三加皮中5种酚酸类成分含量测定。 |
| 关键词: 三加皮 酚酸类 一测多评法 外标法 相对校正因子 |
| DOI：10.13656/j.cnki.gxkx.20211119.002 |
| 投稿时间：2020-05-29 |
| 基金项目:广西重点研发计划项目（桂科AB1850022），南宁市重点研发计划项目（20193117），广西高校中药提取纯化与质量分析重点实验室项目（桂教科研[2014]6号），广西高校中药制剂共性技术研发重点实验室项目（ZJGX2016006，ZJGX201502001），广西壮瑶药工程技术研究中心项目（KJT1900107）和广西中医药大学2019年研究生教育创新计划项目立项课题（YCSY20190037）资助。 |
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| Determination of 5 Phenolic Acid Components in Zhuang Medicine Acanthopanax trifoliatus (L.)Merr. Based on Quantitative Analysis of Multi-component with Single-marker |
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GUO Yongxiu1, JIANG Lin1,2, NONG Yiqing3, LI Fangchan1, SUI Jianing1, WEI Bowei1
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| (1.College of Pharmacy, Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, 530001, China;2.Zhuang Yao Medicine Engineering Technology Center of Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, 530001, China;3.Guangxi Asean Center for Food and Drug Safety Control,Nanning, Guangxi, 530001, China) |
| Abstract: |
| In order to establish a quantitative analysis method of multi-components by single-marker (QAMS) for simultaneous determination of 5 phenolic acid components (neochlorogenic acid, chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C) in Acanthopanax trifoliatus (L.) Merr., chlorogenic acid was used as the internal reference Phenmenex C18 column (4.6 mm×250 mm, 5 μL) was used for gradient elution with acetonitrile (C)-0.2% phosphoric acid (A) as the mobile phase at a flow rate of 1.0 mL/min，column temperature 30℃, detection wavelength 325 nm.The relative correction factors of chlorogenic acid and neocyanogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C were established, and the content of the components to be measured was calculated. The feasibility and accuracy of QAMS were evaluated by comparing the results with those measured by external standard method (ESM). The results showed that the established relative correction factors of neochlorogenic acid, chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C in A. senticosus had good reproducibility. There was no significant difference between the calculated values of QAMS and the measured values of ESM for 5 components in A. trifoliatus (L.)Merr. from 10 habitats.The QAMS method established in this study can be used to determine the contents of 5 phenolic acids in A. trifoliatus (L.)Merr. |
| Key words: Acanthopanax trifoliatus (L.)Merr. phenolic acid QAMS ESM relative correction factors |
