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邱宏聪,赖克道,梁冰,王丽,黄艳,刘布鸣.岗松总黄酮对脂多糖诱导RAW264.7细胞炎症的影响[J].广西科学,2021,28(4):396-401. [点击复制]
- QIU Hongcong,LAI Kedao,LIANG Bing,WANG Li,HUANG Yan,LIU Buming.Effect of Total Flavonoids from Baeckea frutescens on LPS-induced RAW264.7 Cell Inflammation[J].Guangxi Sciences,2021,28(4):396-401. [点击复制]
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| 岗松总黄酮对脂多糖诱导RAW264.7细胞炎症的影响 |
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邱宏聪1,2, 赖克道1, 梁冰1, 王丽1, 黄艳1,2, 刘布鸣1,2
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| (1.广西中医药研究院, 广西南宁 530022;2.广西中药质量标准研究重点实验室, 广西南宁 530022) |
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| 摘要: |
| 为探讨岗松总黄酮（GSH）的抗炎活性机制，通过NF-κB信号通路，研究脂多糖（LPS）诱导RAW264.7细胞炎症的保护作用。实验采用噻唑蓝（MTT）法检测GSH对RAW264.7细胞增殖活性的影响，采用酶联免疫法（ELISA）检测GSH对白介素（IL-1β、IL-8）及转换生长因子β （TGF-β）的影响，采用蛋白印迹法（WB）检测细胞中IκBα蛋白相对表达，采用实时定量基因扩增荧光检测法（QPCR）检测细胞中NF-κB p65的mRNA基因表达。研究结果表明， GSH处理细胞后，可促进RAW264.7细胞增殖（P<0.05），抑制IL-1β、IL-8、TGF-β含量（P<0.01），上调IκBα蛋白的相对表达（P<0.05或P<0.01），抑制NF-κB p65的mRNA基因表达（P<0.01）。推测岗松总黄酮抑制NF-κB信号通路可能是其抗炎活性作用机制之一。 |
| 关键词: 岗松总黄酮 脂多糖 RAW264.7 NF-κB 抗炎 |
| DOI：10.13656/j.cnki.gxkx.20211109.003 |
| 投稿时间：2021-03-15 |
| 基金项目:中国民族医药学会科研项目（2019KYXM-M246-46），广西自然科学基金项目（2014GXNSFBA118175）和广西中药质量标准研究重点实验室开放课题（桂中重开201303）资助。 |
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| Effect of Total Flavonoids from Baeckea frutescens on LPS-induced RAW264.7 Cell Inflammation |
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QIU Hongcong1,2, LAI Kedao1, LIANG Bing1, WANG Li1, HUANG Yan1,2, LIU Buming1,2
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| (1.Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Nanning, Guangxi, 530022, China;2.Guangxi Key Laboratory of Tradtitonal Chinese Medicaine Quality Stardands, Nanning, Guangxi, 530022, China) |
| Abstract: |
| In order to investigate the anti-inflammation activity mechanism of total flavonoids from Baeckea frutescens (GSH)，the protective effect of LPS-induced RAW264.7 cells was studied by NF-κB signaling pathway. MTT method was used to detect the effect of GSH on the proliferation activity of RAW264.7 cells. The effect of GSH on interleukin (IL-1β, IL-8) and transforming growth factor β (TGF-β) was detected by enzyme-linked immunosorbent assay (ELISA). The relative expression of IκBα protein in cells was detected by Western blotting (WB). The mRNA expression of NF-κB p65 in cells was detected by real-time quantitative gene amplification fluorescence assay (QPCR). The results show that after treated by GSH, the proliferation of RAW264.7 cells was promoted (P<0.05), the content of IL-1β、IL-8 and TGF-β were inhibited significantly (P<0.01), the IκBα protein expression was enhanced significantly (P<0.05 or P<0.01), the NF-κB p65 mRNA expression was inhibited significantly (P<0.01). It is speculated that the inhibition of NF-κB signaling pathway by total flavonoids may be one of the anti-inflammatory mechanisms. |
| Key words: total flavonoids from Baeckea frutescens LPS RAW264.7 NF-κB anti-inflammation |
