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  • 朱敏敏,李云鹏,张淼,李晓双,成敏,周珍琼,刘顺梅.岩藻黄素抗D-gal诱导SH-SY5Y细胞衰老作用及机制研究[J].广西科学,2021,28(3):310-320.    [点击复制]
  • ZHU Minmin,LI Yunpeng,ZHANG Miao,LI Xiaoshuang,CHENG Min,ZHOU Zhenqiong,LIU Shunmei.Anti-aging Effect and Mechanism of Fucoxanthin on D-gal Induced SH-SY5Y Cells[J].Guangxi Sciences,2021,28(3):310-320.   [点击复制]
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岩藻黄素抗D-gal诱导SH-SY5Y细胞衰老作用及机制研究
朱敏敏1, 李云鹏1, 张淼1, 李晓双2, 成敏3, 周珍琼1, 刘顺梅1,4
0
(1.潍坊医学院生命科学与技术学院, 山东潍坊 261053;2.潍坊医学院科研处, 山东潍坊 261053;3.潍坊医学院基础医学院, 山东潍坊 261053;4.潍坊医学院医学研究实验中心, 山东潍坊 261053)
摘要:
衰老相关的神经退行性疾病发病率不断增高,严重影响老年人生活质量,为探讨岩藻黄素(Fucoxanthin,FUCO)对神经细胞的抗衰老作用及其机制,采用D-gal诱导人神经母细胞瘤细胞(SH-SY5Y细胞)衰老,然后在细胞培养基中添加FUCO对细胞进行干预,检测其细胞活力、SA-β-半乳糖苷酶活性及细胞内丙二醛(MDA)含量,细胞内自噬体形成及自噬(Autophagy)相关蛋白的表达。研究结果显示,5,10 μmol/L FUCO可明显抑制D-gal诱导的细胞衰老,显著提高SH-SY5Y细胞活力(P<0.05),降低SH-SY5Y细胞内SA-β-半乳糖苷酶的活性(P<0.05)和MDA含量(P<0.05),但20 μmol/L FUCO的抑制作用不明显(P>0.05)。光学显微镜观察结果显示,5,10,20 μmol/L FUCO组细胞内自噬体数量明显比模型组增多;Western blot检测结果显示,与模型组比较,10,20 μmol/L FUCO组自噬相关蛋白ATG5表达增多、LC3Ⅱ/LC3Ⅰ增大、p-mTOR/mTOR减小(P<0.05)。表明FUCO具有抗D-gal诱导的SH-SY5Y细胞衰老的作用,其作用机制可能与适度调节自噬途径有关。
关键词:  岩藻黄素  SH-SY5Y细胞  抗衰老  自噬  D-gal
DOI:10.13656/j.cnki.gxkx.20210608.001
投稿时间:2021-05-07
基金项目:国家自然科学基金面上项目(81870237),山东省自然科学基金面上项目(ZR2020MD094,ZR2010DM010)和山东省大学生科技创新基金项目(S202010438058)资助。
Anti-aging Effect and Mechanism of Fucoxanthin on D-gal Induced SH-SY5Y Cells
ZHU Minmin1, LI Yunpeng1, ZHANG Miao1, LI Xiaoshuang2, CHENG Min3, ZHOU Zhenqiong1, LIU Shunmei1,4
(1.School of Bioscience and Technology, Weifang Medical University, Weifang, Shandong, 261053, China;2.Division of Scientific Research Management, Weifang Medical University, Weifang, Shandong, 261053, China;3.School of Basic Medicine Sciences, Weifang Medical University, Weifang, Shandong, 261053, China;4.Experimental Center for Medical Research, Weifang Medical University, Weifang, Shandong, 261053, China)
Abstract:
The increasing incidence of aging-related neurodegenerative diseases has seriously affected the life quality of the elderly. In order to explore the anti-aging effect of fucoxanthin (FUCO) on the nerve cells and its mechanism, D-gal was used to induce the senescence of human neuroblastoma cell (SH-SY5Y cell), and then FUCO was added to the cell culture medium for the intervention. The cell viability, SA-β-galactosidase activity, intracellular malondialdehyde (MDA) content, intracellular autophagosome formation and autophagy-related protein expression were detected. The results showed that 5 and 10 μmol/L FUCO significantly inhibited D-gal-induced cell senescence and significantly increased SH-SY5Y cell viability (P<0.05), and reduced the intracellular SA-β-galactosidase activity (P<0.05) and the MDA content (P<0.05) in SH-SY5Y cell. But the anti-aging effect of 20 μmol/L fucoxanthin was not significant (P>0.05). The results of optical microscopy showed that the number of intracellular autophagosomes in the cells of the 5, 10, and 20 μmol/L FUCO group was significantly higher than that of the model group. In addition, the results of western blot showed that compared with the model group, the expression of autophagy-related protein ATG5 increased, LC3 Ⅱ/LC3 Ⅰ increased and p-mTOR/mTOR decreased in 10, 20 μmol/L FUCO group (P<0.05). It shows that FUCO has the effect of resisting the senescence of SH-SY5Y cell induced by D-gal, and its mechanism of action may be related to the moderate regulation of autophagy pathway.
Key words:  fucoxanthin  SH-SY5Y cell  anti-aging  autophagy  D-gal

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