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  • 杨齐,黄斌良,吴华德,江朝明.右旋糖酐酶生产菌株的分离鉴定及发酵培养基优化[J].广西科学,2021,28(1):85-91,101.    [点击复制]
  • YANG Qi,HUANG Binliang,WU Huade,JIANG Chaoming.Isolation and Identification of Dextranase-producing Strains and the Optimization of Fermentation Medium[J].Guangxi Sciences,2021,28(1):85-91,101.   [点击复制]
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右旋糖酐酶生产菌株的分离鉴定及发酵培养基优化
杨齐, 黄斌良, 吴华德, 江朝明
0
(广西多得乐生物科技有限公司, 广西南宁 530000)
摘要:
为获得右旋糖酐酶高产菌株,并提高其右旋糖酐酶产量,本研究从土壤中筛选高产右旋糖酐酶真菌,并采用响应面法对其发酵培养基进行优化。实验筛选得到一株生产右旋糖酐酶的菌株DJ72,经鉴定为腐皮镰刀菌(Fusarium solani),并确定其最佳发酵培养基配方为右旋糖酐T2000 11.88 g/L、蛋白胨7.15 g/L、尿素 3.14 g/L、磷酸氢二钾1.50 g/L、硫酸镁0.20 g/L、硫酸亚铁0.05 g/L,经优化后右旋糖酐酶活力最高可达228 U/mL。本研究为右旋糖酐酶的工业化应用提供可能。
关键词:  右旋糖酐  右旋糖酐酶  腐皮镰刀菌  响应面  培养基优化
DOI:10.13656/j.cnki.gxkx.20210309.009
基金项目:南宁市科学研究与技术开发项目(20153330)资助。
Isolation and Identification of Dextranase-producing Strains and the Optimization of Fermentation Medium
YANG Qi, HUANG Binliang, WU Huade, JIANG Chaoming
(Guangxi Duodele Biotechnology Co., Ltd., Nanning, Guangxi, 530000, China)
Abstract:
In order to obtain a high-yield dextranase strain and increase its dextranase production,this article screened high-yield dextranase fungi from the soil and optimized its fermentation medium using Response Surface method. A strain DJ72 that produces dextranase was obtained through experimental screening,which was identified as Fusarium solani. The optimal formula of fermentation medium was determined as follows:Dextran T2000 11.88 g/L,peptone 7.15 g/L,urea 3.14 g/L,dipotassium phosphate 1.50 g/L,magnesium sulfate 0.20 g/L,ferrous sulfate 0.05 g/L. After optimization,the highest activity of dextranase can reach 228 U/mL. This study provides the possibility for the industrial application of dextranase.
Key words:  dextran  dextranase  Fusarium solani  response surface  medium optimization

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