| 引用本文： |
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林玉坤,谢春兰,贾凌云,张改云,杨献文,潘英妮.珊瑚来源放线菌Streptomyces albidoflavus M13.1的次级代谢产物化学成分研究[J].广西科学,2020,27(5):564-569. [点击复制]
- LIN Yukun,XIE Chunlan,JIA Lingyun,ZHANG Gaiyun,YANG Xianwen,PAN Yingni.Study on Chemical Constituents of the Secondary Metabolites of Coral-derived Actinomycete Streptomyces albidoflavus M13.1[J].Guangxi Sciences,2020,27(5):564-569. [点击复制]
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| 摘要: |
| 为了研究珊瑚来源放线菌Streptomyces albidoflavus M13.1中的次级代谢产物，利用硅胶色谱法、凝胶色谱法、薄层色谱法、中压液相色谱法等技术对珊瑚来源放线菌Streptomyces albidoflavus M13.1发酵物的乙酸乙酯提取物进行分离和纯化，通过核磁共振氢谱（1H-NMR）、核磁共振碳谱（13C-NMR）、质谱（MS）等波谱数据及文献对照方法对化合物进行结构鉴定。从放线菌S.albidoflavus M13.1分离鉴定出13个化合物，分别为5-（6-methyl-7-oxooctyl）furan-2（5H）-one（1）、肉桂酸（2）、环（亮-脯）二肽（3）、5-（6，7-dihydroxy-6-methyloctyl）furan-2（5H）-one（4）、环（丙-脯）二肽（5）、香豆酸（6）、环（丙-亮）二肽（7）、N-乙酰基酪胺（8）、环（4-羟基-脯-苯丙）二肽（9）、环（甘-丙）二肽（10）、环（甘-脯）二肽（11）、尿嘧啶核苷（12）、2'-O-甲氧基尿嘧啶核苷（13）。这13个化合物均为首次从珊瑚来源的S.albidoflavus M13.1样品中分离得到，且均无明显的抗肿瘤细胞增殖活性。 |
| 关键词: 珊瑚 放线菌 次级代谢产物 提取分离 结构鉴定 |
| DOI：10.13656/j.cnki.gxkx.20201214.010 |
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| 基金项目:国家自然科学基金项目（41676130）资助。 |
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| Study on Chemical Constituents of the Secondary Metabolites of Coral-derived Actinomycete Streptomyces albidoflavus M13.1 |
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LIN Yukun1,2, XIE Chunlan2, JIA Lingyun1, ZHANG Gaiyun2, YANG Xianwen2, PAN Yingni1
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| (1.School of Traditional Chinese Medicine of Shenyang Pharmaceutical University, Shenyang, Liaoning, 110016, China;2.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, Fujian, 361005, China) |
| Abstract: |
| In order to study the secondary metabolites of the coral-derived actinomycetes Streptomyces albidoflavus M13.1,silica gel chromatography,gel chromatography,thin-layer chromatography,medium pressure liquid chromatography and other techniques were used to separate and purify the ethyl acetate extract of the coral-derived actinomyces S.albidoflavus M13.1.The structure of the compound was identified by spectral data such as proton nuclear magnetic resonance spectroscopy (1H-NMR),carbon nuclear magnetic resonance (13C-NMR),mass spectrometry (MS) and literature comparison methods.13 compounds were isolated and identified from the actinomyces S.albidoflavus M13.1,which are 5-(6-methyl-7-oxooctyl)furan-2(5H)-one (1),cinnamic acid (2),cyclo-(Leu-Pro) (3),5-(6,7-dihydroxy-6-methyloctyl)furan-2(5H)-one (4),cyclo-(Ala-Pro) (5),coumaric acid (6),cyclo-(Ala-Leu) (7),N-acetyltyramine (8),cyclo-(4-OH-Pro-Phe) (9),cyclo-(Gly-Ala) (10),cylco-(Gly-Pro) (11),uridine (12),and 2'-O-methoxyluridine (13).All 13 compounds were isolated for the first time from the S.albidoflavus M13.1.These 13 compounds were all isolated from the sample of coral-derived S.albidoflavus M13.1 for the first time. |
| Key words: coral actinomycetes secondary metabolites extraction and isolation identification of structures |
