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孙博,霍华珍,蔡爱华,谢运昌,李典鹏.不同产地大果山楂总黄酮含量及抗氧化活性[J].广西科学,2020,27(4):356-361. [点击复制]
- SUN Bo,HUO Huazhen,CAI Aihua,XIE Yunchang,LI Dianpeng.Total Flavonoids Content and Antioxidant Activity of Malus doumeri Fruit from Different Producing Areas[J].Guangxi Sciences,2020,27(4):356-361. [点击复制]
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| 不同产地大果山楂总黄酮含量及抗氧化活性 |
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孙博1,2, 霍华珍2, 蔡爱华2, 谢运昌2, 李典鹏1,2
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| (1.桂林理工大学化学与生物工程学院, 广西桂林 541004;2.广西壮族自治区中国科学院广西植物研究所, 广西植物功能物质研究与利用重点实验室, 广西桂林 541006) |
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| 摘要: |
| 为测定不同产地大果山楂总黄酮含量及其体外抗氧化活性，选用广西、广东10种不同产地的大果山楂鲜果，采用回流提取法和超声提取法对大果山楂总黄酮进行提取；对可见分光光度法进行方法学考察，并采用DPPH自由基清除法和氧自由基吸收法来评价大果山楂体外抗氧化活性。结果表明，超声提取法耗时短、效率高、操作简便，优于回流提取法，适用于大果山楂总黄酮的提取；芦丁在0.008—0.064 mg/mL范围内线性关系良好，R2=0.998 3，平均加样回收率为99.78%（RSD=1.07%），表明可见分光光度法适用于大果山楂总黄酮含量测定；10种产地大果山楂总黄酮含量存在显著差异（P<0.05），其中广西靖西市大果山楂总黄酮含量高达38.61 mg/g，大部分产地的大果山楂总黄酮含量为20.91—32.63 mg/g。体外抗氧化活性结果表明，不同产地大果山楂总黄酮提取液均具有良好的DPPH自由基清除能力和氧自由基吸收能力，以广西靖西市大果山楂总黄酮提取液体外抗氧化效果最佳，其DPPH自由基清除能力的半数清除浓度IC50=（0.048±0.006）mg/mL，氧自由基吸收能力为（39.35±0.42）mg TE/g FW。本研究为大果山楂的原料、产品质量控制，以及功能性食品的开发利用提供一定的理论基础。 |
| 关键词: 大果山楂 不同产地 总黄酮 含量测定 抗氧化活性 |
| DOI：10.13656/j.cnki.gxkx.20200924.003 |
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| 基金项目:广西科技重大专项（桂科AA17204038），广西自然科学基金项目（2017GXNSFAA198009）和广西植物功能物质研究与利用重点实验室主任基金项目（ZRJJ2018-2）资助。 |
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| Total Flavonoids Content and Antioxidant Activity of Malus doumeri Fruit from Different Producing Areas |
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SUN Bo1,2, HUO Huazhen2, CAI Aihua2, XIE Yunchang2, LI Dianpeng1,2
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| (1.College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, Guangxi, 541004, China;2.Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China) |
| Abstract: |
| In order to determine the total flavonoids content and in vitro antioxidant activity of Malus doumeri fruits from different producing areas, the fresh fruits of M.doumeri from 10 different places in Guangxi and Guangdong were selected, and the total flavonoids extracted by reflux extraction method and ultrasonic extraction method. Methodological investigation was given to visible spectrophotometry. DPPH free radical and oxygen free radical absorption method were adopted to evaluate the in vitro antioxidant activity of M.doumeri fruits. The results showed that compared with the reflux extraction, the ultrasonic extraction was shorter in time, higher in efficiency, and simpler in operation. It was suitable for the extraction of total flavonoids in M.doumeri fruits. Within the range from 0.008 mg/mL to 0.064 mg/mL, the rutin had a good linear relationship, its R2 was 0.998 3, and the average recovery rate was 99.78% (RSD=1.07%), which showed that the visible spectrophotometry was suitable for the determination of total flavonoids in M.doumeri fruit. There were significant differences in the total flavonoids of M.doumeri fruit in 10 producing areas (P<0.05). Among them, the total flavonoids content of M.doumeri fruit in Jingxi City of Guangxi was as high as 38.61 mg/g. And in most places the total flavonoids of M.doumeri fruit was between 20.91 mg/g and 32.63 mg/g. The in vitro antioxidant activity results showed that the total flavonoid extract solution of M.doumeri fruits from different production places had good DPPH free radical scavenging ability and oxygen free radical absorption ability. The total flavonoids extract solution of M.doumeri fruits from Jingxi City of Guangxi had the best in vitro antioxidant effect. Its DPPH free radical scavenging ability was as follows: IC50=(0.048±0.006) mg/mL, and the absorption capacity of oxygen free radicals was as follows:(39.35±0.42) mg TE/g FW. This study can provide a certain theoretical basis for the quality control of raw materials and products of M.doumeri fruit and the development and utilization of functional food. |
| Key words: Malus doumeri fruit different producing areas total flavonoids content test antioxidant activity |
