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刘雯,郭海姣,刘进宝,覃洁萍,胡华,黄盛梅,王先军.HPLC法同时测定外感风痧颗粒中4种成分[J].广西科学,2019,26(5):539-542. [点击复制]
- LIU Wen,GUO Haijiao,LIU Jinbao,QIN Jieping,HU Hua,HUANG Shengmei,WANG Xianjun.Simultaneous determination of four components in Waiganfengsha granule by HPLC[J].Guangxi Sciences,2019,26(5):539-542. [点击复制]
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| HPLC法同时测定外感风痧颗粒中4种成分 |
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刘雯1, 郭海姣1, 刘进宝2, 覃洁萍1, 胡华3, 黄盛梅1, 王先军1
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| (1.广西中医药大学药学院, 广西南宁 530001;2.广西国际壮医医院, 广西南宁 530200;3.广西中医药大学制药厂, 广西南宁 530023) |
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| 摘要: |
| 本文建立同时测定外感风痧颗粒中原儿茶酸、原儿茶醛、绿原酸、咖啡酸4种成分的HPLC方法。色谱柱为依利特Hypersil C18柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.2%磷酸，梯度洗脱，流速1.0 mL·min-1；柱温25℃。结果显示：4种指标成分分离效果良好，原儿茶酸在3.672～33.048 μg·mL-1、原儿茶醛在1.236～11.124 μg·mL-1、绿原酸在9.2～82.8 μg·mL-1、咖啡酸在1.488～13.392 μg·mL-1线性关系良好；平均加样回收率均为97.6%～103.3%。本方法准确、灵敏、可靠，重现性较好，可用于外感风痧颗粒的质量控制。 |
| 关键词: 外感风痧颗粒 HPLC 原儿茶酸 原儿茶醛 绿原酸 咖啡酸 |
| DOI：10.13656/j.cnki.gxkx.20191113.002 |
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| 基金项目:广西高校中药提取纯化与质量分析重点实验室项目（桂教科研[2014]6）和广西高校壮医方药基础与应用研究重点实验室（厅级）科研课题（桂教科研[2016]6 zyfy201608）资助。 |
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| Simultaneous determination of four components in Waiganfengsha granule by HPLC |
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LIU Wen1, GUO Haijiao1, LIU Jinbao2, QIN Jieping1, HU Hua3, HUANG Shengmei1, WANG Xianjun1
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| (1.College of Pharmacy, Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, 530001, China;2.International Zhuang Medical Hospital of Guangxi, Nanning, Guangxi, 530200, China;3.Pharmaceutical Factory, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530023, China) |
| Abstract: |
| In this paper,an HPLC method was established for the simultaneous determination of four components of protocatechuic acid,protocatechuic aldehyde,chlorogenic acid and caffeic acid in Waiganfengsha granule.The chromatographic separation was achieved on a Hypersil C18 (250 mm×4.6 mm,5 μm) column with acetonitrile -0.2% phosphoric acid as mobile phases for gradient elution,at the flow rate of 1.0 mL·min-1.The column temperature was 25℃.The results showed that the four active components were well separated and showed good linear relationship when protocatechuic acid was 3.672-33.048 μg·mL-1,protocatechuic aldehyde was 1.236-11.124 μg·mL-1,chlorogenic acid was 9.2-82.8 μg·mL-1,and caffeic acid was 1.488-13.392 μg·mL-1.The average recovery rates were all between 97.6% and 103.3%.The method is accurate,sensitive,credible and repeatable and can be applied to the quality control of Waiganfengsha granule. |
| Key words: Waiganfengsha granule HPLC protocatechuic acid protocatechuic aldehyde chlorogenic acid caffeic acid |
