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覃香香,周玉恒,陈海珊,王磊,蔡爱华,韦逸飞,姜瑞涛.响应面法优化玉米苞叶中木聚糖的提取工艺[J].广西科学,2019,26(2):222-227. [点击复制]
- QIN Xiangxiang,ZHOU Yuheng,CHEN Haishan,WANG Lei,CAI Aihua,WEI Yifei,JIANG Ruitao.Optimization of Extraction Process for Xylan from Corn Bracts by Response Surface Methodology[J].Guangxi Sciences,2019,26(2):222-227. [点击复制]
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| 响应面法优化玉米苞叶中木聚糖的提取工艺 |
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覃香香1, 周玉恒1, 陈海珊1, 王磊1, 蔡爱华1, 韦逸飞2, 姜瑞涛3
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| (1.广西壮族自治区中国科学院广西植物研究所, 广西木质纤维素生物炼制工程技术研究中心, 广西桂林 541006;2.桂林医学院, 广西桂林 541004;3.桂林理工大学, 广西桂林 541006) |
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| 摘要: |
| 为了给玉米苞叶工业化生产木聚糖提供基础研究资料，本研究以玉米苞叶为原料，采用响应面法（RSM）对玉米苞叶木聚糖碱法提取工艺进行优化研究，选择碱浓度、提取温度和提取时间作为考察因素，以玉米苞叶木聚糖提取率作为评价指标，考察3个不同因素及其交互作用对玉米苞叶木聚糖提取率的影响。研究结果表明：玉米苞叶木聚糖的最佳提取工艺条件为氢氧化钠浓度9.2%（M/V）、提取温度93.0℃、提取时间13.0 h；在此工艺参数条件下提取3批玉米苞叶，获得的木聚糖平均提取率为（15.8±1.2）%（n=3），与采用响应面法优化回归模型预测提取率（16.0%）基本一致。 |
| 关键词: 玉米苞叶 木聚糖 响应面法 高效液相(HPLC) |
| DOI：10.13656/j.cnki.gxkx.20190506.005 |
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| 基金项目:广西功能物质研究与利用重点实验室主任基金项目（ZRJJ2016-18，ZR2017-6），广西植物研究所基本业务费项目（桂植业201701，桂植业18012）和广西自然科学基金项目（2017GXNSFAA198009，2018GXNSFAA050093）资助。 |
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| Optimization of Extraction Process for Xylan from Corn Bracts by Response Surface Methodology |
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QIN Xiangxiang1, ZHOU Yuheng1, CHEN Haishan1, WANG Lei1, CAI Aihua1, WEI Yifei2, JIANG Ruitao3
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| (1.Guangxi Lignocellulose Biorefinery Engineering Technology Research Center, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China;2.Guilin Medical University, Guilin, Guangxi, 541004, China;3.Guilin University of Technology, Guilin, Guangxi, 541006, China) |
| Abstract: |
| In order to provide basic research data for the industrial production of xylan from corn glutinous rice leaves, this study used corn bracts as raw material to optimize the alkali extraction of xylan from corn bract by the response surface methodology (RSM). NaOH concentration, extraction temperature and extraction time were selected as the influencing factors, and the extraction rate of xylan was evaluated as the evaluation index. The effects of 3 different factors and their interactions on the extraction rate of xylan from corn bract were investigated. The results indicated that the optimal xylan extraction conditions from corn bracts were NaOH concentration of 9.2% (M/V), extraction temperature of 93.0℃ and extraction time of 13.0 h, respectively. Three batches of corn bract were extracted under the condition of this process parameter. The average extraction rate of xylan obtained was (15.8±1.2)% (n=3), which was basically consistent with the predicted extraction rate (16.0%) by response surface methodology. |
| Key words: corn bract xylan response surface methodology HPLC |
