| 引用本文： |
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俸雪,严睿萍,沈峰林,王小香,庞宗文.以甘薯为原料发酵生产丙酮-丁醇-乙醇的研究[J].广西科学,2018,25(3):330-338. [点击复制]
- FENG Xue,YAN Ruiping,SHEN Fenglin,WANG Xiaoxiang,PANG Zongwen.Research on Fermentation of Acetone-Butanol-Ethanol with Sweet Potato as Raw Material[J].Guangxi Sciences,2018,25(3):330-338. [点击复制]
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| 摘要: |
| [目的]研究以甘薯为原料发酵生产丙酮-丁醇-乙醇(ABE)的可行性。[方法]以本研究分离鉴定的丙酮丁醇梭菌(Clostridium acetobutylicum)J-3-3为生产菌株,通过发酵培养基和发酵条件的优化,确定最佳的发酵工艺条件。发酵产物采用气相色谱的内标法测定。[结果]最佳发酵培养基组成:甘薯液化液初糖浓度为100 g/L,豆粕8 g/L,KH2PO4 0.8 g/L,FeSO4·7H2O 0.06 g/L。最佳发酵条件:温度37℃,初始pH自然(pH值为5.8),种龄22 h,接种量10%。在此发酵工艺条件下发酵60 h,溶剂产量达到最大,丙酮、乙醇和丁醇的产量分别为6.98 g/L、1.16 g/L和15.34 g/L,总溶剂23.48 g/L。[结论]研究结果表明,以甘薯为原料发酵生产丙酮-丁醇-乙醇是可行的,这为拓宽发酵法生产丙酮-丁醇-乙醇的原料来源以及甘薯的深加工提供了新的思路。 |
| 关键词: 丙酮丁醇梭菌 丙酮-丁醇-乙醇发酵 甘薯 |
| DOI：10.13656/j.cnki.gxkx.20180528.004 |
| 投稿时间：2018-04-12 |
| 基金项目:国家"863"计划项目(2011AA02A208)和广西科学基金项目(桂科字0991037)资助 |
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| Research on Fermentation of Acetone-Butanol-Ethanol with Sweet Potato as Raw Material |
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FENG Xue, YAN Ruiping, SHEN Fenglin, WANG Xiaoxiang, PANG Zongwen
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| (College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China) |
| Abstract: |
| [Objective] The feasibility of producing acetone-butanol-ethanol (ABE) from sweet potato as raw material was studied.[Methods] ABE fermentation was carried out with Clostridium acetobutylicum J-3-3 which was isolated and identified in this study.The optimum fermentation process was determined by optimizing the fermentation medium and the fermentation conditions.Fermentation products were determined by gas chromatography internal standard method.[Results] The optimum medium composition:The initial concentration of sugar in sweet potato liquor was 100 g/L, soybean meal was 8 g/L, KH2PO4 was 0.8 g/L, FeSO4·7H2O was 0.06 g/L, and the initial pH was 7.5.The optimum fermentation conditions were as follows:Temperature 37℃, initial pH (pH 5.8), seedling age 22 h, and inoculation amount 10%.Under this fermentation condition, the solvent yield reached the maximum after 60 h of fermentation.The yields of acetone, ethanol and butanol were 6.98 g/L, 1.16 g/L and 15.34 g/L, respectively.The total solvent was 23.48 g/L.[Conclusion] The research result demonstrated that it was feasible to perform the ABE fermentation with sweet potato as raw material, which provides a new idea of broadening the source of raw material for producing acetone-butanol-ethanol from fermentation and deep processing of sweet potato. |
| Key words: Clostridium acetobutylicum acetone-butanol-ethanol fermentation sweet potato |
