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潘勤春,刘兴胥,吕军,朱婧,王青艳,周礼芹.重组脂肪酶自诱导发酵培养基优化[J].广西科学,2018,25(3):299-303. [点击复制]
- PAN Qinchun,LIU Xingxu,LV Jun,ZHU Jing,WANG Qingyan,ZHOU Liqin.Optimization of auto-induction fermentation medium for recombined lipase[J].Guangxi Sciences,2018,25(3):299-303. [点击复制]
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| 重组脂肪酶自诱导发酵培养基优化 |
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潘勤春1, 刘兴胥1, 吕军1, 朱婧2, 王青艳2, 周礼芹1,2
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| (1.南宁市新科健生物技术有限责任公司, 广西南宁 530003;2.广西科学院, 广西南宁 530007) |
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| 摘要: |
| [目的]优化自动诱导发酵培养基,提高脂肪酶产量。[方法]通过对构建的一株转座子整合型脂肪酶重组菌株,采用自动诱导发酵表达方法,并结合CCD响应面实验设计方法,对自动诱导培养基中的碳源进行优化,获得了1个二次模型用于描述发酵培养基中碳源对产脂肪酶的影响。[结果]优化后的最适自动诱导培养基(W/V)组成为glycerol 2.596,glucose 0.035,lactose 1.289,tryptone 1.0,yeast extract 0.5,Na2HPO4·12H2O 1.79,KH2PO4 0.68,NH4Cl 0.267 5,Na2SO4 0.071,MgSO4·7H2O 0.05。[结论]发酵试验表明,最优碳源培养基的比活力为R1=6.35 U/mg,比初始发酵培养基提高近3倍。 |
| 关键词: 脂肪酶 自动诱导 响应面 培养基优化 |
| DOI：10.13656/j.cnki.gxkx.20180612.002 |
| 投稿时间：2018-04-28 |
| 基金项目:广西科学研究与技术开发计划项目(桂科合15104001-18)资助 |
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| Optimization of auto-induction fermentation medium for recombined lipase |
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PAN Qinchun1, LIU Xingxu1, LV Jun1, ZHU Jing2, WANG Qingyan2, ZHOU Liqin1,2
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| (1.Nanning Newkergen Biotechnology Sciences Co. Ltd., Nanning, Guangxi, 530003, China;2.Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| [Objective] Optimizing the auto-induction of the fermentation medium to increase lipase enzyme production.[Methods] In this research, through the construction of a transposon-integrated lipase recombinant bacterial strain, using automatic induction fermentation expression method combined with CCD response surface experimental design method, the carbon source in the auto-induction medium was optimized, and a quadratic model was obtained, which was used to describe the effect of the carbon source in the fermentation medium on lipase production.[Results] The optimized fermentation medium (W/V) contained glycerol 2.596, glucose 0.035, lactose 1.289, tryptone 1.0, yeast extract 0.5, Na2HPO4·12H2O 1.79, KH2PO4 0.68, NH4Cl 0.267 5, Na2SO40.071, MgSO4·7H2O 0.05.[Conclusion] The results of the fermentation test confirmed that the lipase enzyme specific activity in the optimized fermentation medium was 6.35 U/mg, which was nearly three times higher than that in the initial fermentation medium. |
| Key words: lipase auto-induction response surface method medium optimization |
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