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李鹏飞,余庆,李菲,覃仙玲,董德信,陈宪云,牙韩争,柯珂,秦启伟.基于新型核酸适配体-荧光分子检测探针的石斑鱼虹彩病毒病快速诊断[J].广西科学,2018,25(1):63-67. [点击复制]
- LI Pengfei,YU Qing,LI Fei,QIN Xianling,DONG Dexin,CHEN Xianyun,YA Hanzheng,KE Ke,QIN Qiwei.Establishment and Characterization of Novel Specific Aptamer-based Fluorescent Molecular Probe for Rapid Diagnosis of Grouper Iridovirus Diseases[J].Guangxi Sciences,2018,25(1):63-67. [点击复制]
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| 基于新型核酸适配体-荧光分子检测探针的石斑鱼虹彩病毒病快速诊断 |
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李鹏飞1, 余庆1, 李菲1, 覃仙玲1, 董德信1, 陈宪云1, 牙韩争1, 柯珂1, 秦启伟2
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| (1.广西科学院广西北部湾海洋研究中心, 广西近海海洋环境科学重点实验室, 广西南宁 530007;2.华南农业大学海洋学院, 广东广州 510642) |
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| 摘要: |
| [目的]石斑鱼虹彩病毒(Singapore grouper iridovirus,SGIV)是引起华南沿海地区重要海水养殖鱼类石斑鱼(Epinephelus tauvina)发生病毒性鱼病的主要病毒性病原之一,其引起的鱼病具有发病迅速、死亡率高、流行面广等特点,严重威胁华南地区石斑鱼养殖业的健康可持续发展。着力发展操作便捷、成本低、耗时短、准确度高的SGIV快速检测技术,对于及早发现、确定病原,进而有的放矢地制定治疗方案来控制病原扩散、降低损失至关重要。[方法]基于核酸适配体(Q2)就SGIV病的快速检测诊断技术进行系统研究,开发出一种新型的核酸适配体-荧光分子检测探针(Aptamer Q2-based fluorescent molecular probe,Q2-AFMP),并对Q2-AFMP检测SGIV感染的特异性、灵敏性和稳定性进行分析。[结果]Q2-AFMP可以特异性检测SGIV感染,且其灵敏性和稳定性均比较高。[结论]本研究中基于核酸适配体构建的新型高特异性荧光分子探针(Q2-AFMP)有望实现对海水养殖中石斑鱼虹彩病毒病的快速诊断、实时监控和有效预防。 |
| 关键词: 石斑鱼虹彩病毒 核酸适配体-荧光分子探针 高特异性和灵敏性 快速诊断 |
| DOI：10.13656/j.cnki.gxkx.20180206.004 |
| 投稿时间：2017-12-06修订日期：2018-01-08 |
| 基金项目:国家自然科学基金项目(41706145),广西自然科学基金项目(2017GXNSFBA198176)和广西科学院基本科研业务费项目(2017YJJ23002)资助 |
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| Establishment and Characterization of Novel Specific Aptamer-based Fluorescent Molecular Probe for Rapid Diagnosis of Grouper Iridovirus Diseases |
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LI Pengfei1, YU Qing1, LI Fei1, QIN Xianling1, DONG Dexin1, CHEN Xianyun1, YA Hanzheng1, KE Ke1, QIN Qiwei2
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| (1.Guangxi Key Laboratory of Marine Environmental Science, Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, 510642, China) |
| Abstract: |
| [Objective] Grouper iridovirus is the major virus pathogen in South China, and causes high mortality to marine cultured grouper, which seriously threatens the healthy and sustainable development for grouper mariculture.Then effective detective techniques for the rapid diagnosis of SGIV infection are urgently needed.[Methods] Based on the nucleotide aptamer (Q2), the rapid detection and diagnosis technology of SGIV disease was systematically studied and a new Aptamer Q2-based fluorescent molecular probe (Q2-AFMP) was developed.The specificity, sensitivity and stability of Q2-AFMP in detecting SGIV infection were analyzed.[Results] Aptamer Q2-Based fluorescent molecular probe (Q-AFMP)could detect SGIV infection with high specificity, sensitivity and stability.[Conclusion] The novel fluorescent molecular probe (Q2-AFMP)holds great potentials for rapid early diagnosis of pathogen SGIV infection in grouper mariculture in South China. |
| Key words: Singapore grouper iridovirus Q2-AFMP high specificity and sensitivity rapid early diagnosis |
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