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宋瑾,周利琴,林钻煌,覃青云,伍善广,许静,孙建华,廖丹葵.中草药提取物体外止血效果评价模型[J].广西科学,2017,24(4):407-412. [点击复制]
- SONG Jin,ZHOU Liqin,LIN Zuanhuang,QIN Qingyun,WU Shanguang,XU Jing,SUN Jianhua,LIAO Dankui.Establishment and Application of the Model of External Hemostatic of Herb[J].Guangxi Sciences,2017,24(4):407-412. [点击复制]
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| 中草药提取物体外止血效果评价模型 |
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宋瑾1, 周利琴1, 林钻煌2, 覃青云2, 伍善广1,3, 许静2, 孙建华1, 廖丹葵1
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| (1.广西大学化学化工学院, 广西高校资源化工应用新技术重点实验室, 广西南宁 530004;2.柳州两面针股份有限公司, 广西柳州 545001;3.广西科技大学, 广西柳州 545006) |
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| 摘要: |
| [目的]建立体外综合止血评价模型,筛选止血效果良好的中草药。[方法]选择凝血酶时间(Thrombin time,TT)、血小板聚集率(Platelet aggregation rate,PAgR)和黏附率(Platelet adhesion rate,PAdR)、抗凝血酶Ⅲ(Anti-thrombin Ⅲ activity,AT-Ⅲ)活性、血浆复钙时间(Recovery calcium time,RT)、最低抑菌浓度(MIC)、最低杀菌浓度(MBC)7项指标,应用层次分析法建立分析评价模型,对侧柏叶、三七、两面针、止血环酸进行体外止血活性评价,并通过小鼠出血与凝血实验验证模型的可行性。[结果]模型分析表明止血效果好的为侧柏叶、三七、止血环酸;效果较好的为两面针;效果差的为生理盐水。模型分析结果与动物实验结果相吻合。[结论]该模型对中草药止血效果评价方法的建立有一定的参考价值。 |
| 关键词: 中草药提取物 止血 抗菌 评价模型 |
| DOI：10.13656/j.cnki.gxkx.20170516.002 |
| 投稿时间：2017-02-10修订日期：2017-03-15 |
| 基金项目:广西科学研究与技术开发计划项目(1355012-4)和柳州市科技攻关项目(2013C040403)资助。 |
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| Establishment and Application of the Model of External Hemostatic of Herb |
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SONG Jin1, ZHOU Liqin1, LIN Zuanhuang2, QIN Qingyun2, WU Shanguang1,3, XU Jing2, SUN Jianhua1, LIAO Dankui1
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| (1.Key Laboratory of New Technology Applied in Chemical Engineering, College of Chemistry and Chemical Engineering of Guangxi University, Nanning, Guangxi, 530004, China;2.LMZ Limited by Share Ltd, Liuzhou, Guangxi, 545001, China;3.Guangxi University of Science and Technology, Liuzhou, Guangxi, 545006, China) |
| Abstract: |
| [Objective] To establish a comprehensive evaluation model for the selection of the hemostatic activity of herb.[Methods] Thrombin time, platelet aggregation rate and the adhesion rate, anti-thrombin Ⅲ activity and plasma recovery calcium time, minimum inhibitory concentration, minimum bactericidal concentrations were tested for evaluation of in vitro hemostatic activity.Analytic hierarchy process was used to establish the evaluation system of hemostasis in vitro, and the feasibility of the model is verified by the experiment of hemorrhage and coagulation of mice.[Results] The model analysis showed that the hemostatic effect was good for Platycladus orientalis leaf, Panax notoginseng, tranexamic acid;better for Radix zanthoxyli;poor for physiological saline.The results were consistent with the experimental results of the animals.[Conclusion] The model has a certain reference value for the establishment of the evaluation method of herbal hemostasis. |
| Key words: herbal extracts hemostatic antibacterial evaluation model |
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