| 引用本文： |
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武志超,杨欢东,何杜鹃,宋晓勇,周化斌,杨海龙.促进剂对木耳液体发酵生产的影响[J].广西科学,2017,24(4):376-381,388. [点击复制]
- WU Zhichao,YANG Huandong,HE Dujuan,SONG Xiaoyong,ZHOU Huabin,YANG Hailong.Effects of Stimulators on the Liquid Fermentation of Auricularia auricula[J].Guangxi Sciences,2017,24(4):376-381,388. [点击复制]
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| 摘要: |
| [目的]有效地促进木耳菌体生长及胞外多糖的生产,确定可显著提高木耳液体发酵生产的促进剂及其添加量。[方法]在木耳液体发酵培养基中分别添加20种不同的促进剂,每种促进剂设置4个浓度,发酵6 d后检测发酵液中的菌丝体、胞外多糖含量。然后对促进效果较好的Tween40、硬脂酸、棕榈酸、油酸进行正交试验。[结果]单因素试验结果表明,0.1%的棕榈酸、1%的硬脂酸、0.5%的Tween40、0.1%的油酸均可显著促进木耳胞外多糖的生产。正交试验结果表明,在发酵培养基中添加剂最佳组合为0.5% Tween40、1.5%硬脂酸、0.1%棕榈酸、0.25%油酸。[结论]硬脂酸、棕榈酸、油酸和Tween40可显著促进木耳胞外多糖的发酵生产。 |
| 关键词: 木耳 生物量 多糖 促进剂 发酵生产 |
| DOI：10.13656/j.cnki.gxkx.20170731.001 |
| 投稿时间：2017-01-05修订日期：2017-02-23 |
| 基金项目:浙江省科技计划项目(2015C32131)和温州市科技计划项目(N20140045)资助。 |
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| Effects of Stimulators on the Liquid Fermentation of Auricularia auricula |
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WU Zhichao, YANG Huandong, HE Dujuan, SONG Xiaoyong, ZHOU Huabin, YANG Hailong
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| (School of Life & Environmental Science, Wenzhou University, Wenzhou, Zhejiang, 325035, China) |
| Abstract: |
| [Objective] To effectively enhance the mycelia growth and extracellular polysaccharide production of Auricularia auricula, stimulators for liquid fermentation of A.auricula were screened.[Methods] Twenty stimulators were respectively added to the fermentation media at four different concentrations.The extracellular polysaccharide and mycelia biomass were determined after 6 d of fermentation.Moreover, Tween40, stearic acid, palmitic acid and oleic acid were selected for orthogonal experiment.[Results] 0.1% of palmitic acid, 1% of stearic acid, 0.5% of Tween40 and 0.1% of oleic acid in the culture medium significantly increased the yield of extracellular polysaccharide. Orthogonal experiment results showed that the optimal stimulator combination in the fermentation media was 0.5% Tween40, 1.5% stearic acid, 0.1% palmitic acid, 0.25% oleic acid.[Conclusion] Stearic acid, palmitic acid, oleic acid and Tween40 could promote the extracellular polysaccharide bio-synthesis in submerged culture of A.auricula. |
| Key words: Auricularia auricular biomass polysaccharide stimulator fermentation production |
