| 引用本文： |
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杨静文,孔繁思,栾霞,高仁钧.嗜热糖苷酶与酶催化糖化合物合成研究进展[J].广西科学,2017,24(1):54-60. [点击复制]
- YANG Jingwen,KONG Fansi,LUAN Xia,GAO Renjun.The Thermostable Glycosidase and Enzymatic Synthesis of Glycoconjugates: Current State and Perspectives[J].Guangxi Sciences,2017,24(1):54-60. [点击复制]
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| 摘要: |
| 本文对近年来关于嗜热糖苷酶的研究进行了全面的论述,包括嗜热糖苷酶基因的克隆与表达的现状以及在酶法合成糖苷类化合物方面的应用,对比分析了目前提高转糖苷效率的方法,如定点突变、合理设计溶液体系、微波辐射等。在糖苷类化合物的水解与合成的工业生产中,嗜热糖苷酶以其良好的热稳定性可望成为一类高效、稳定的工具酶。 |
| 关键词: 糖苷酶 克隆 表达 糖苷 合成 突变 离子液体 微波辐射 |
| DOI：10.13656/j.cnki.gxkx.20170119.004 |
| 投稿时间：2016-12-15 |
| 基金项目:国家"863"高科技项目(2013AA102104)资助 |
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| The Thermostable Glycosidase and Enzymatic Synthesis of Glycoconjugates: Current State and Perspectives |
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YANG Jingwen1, KONG Fansi1, LUAN Xia2, GAO Renjun1
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| (1.Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education, School of Life Science, Jilin University, Changchun, Jilin, 130012, China;2.Academy of State Administration of Grain, Beijing, 100037, China) |
| Abstract: |
| In this paper, the progress of thermostable glycosidases in recent years has been summarized in detail, including the current state of cloning and expression of glycosidases and applications in enzymatic synthesis of glycosidic compounds. The methods of improving the efficiency of transglycosylation, such as site directed mutagenesis, rational design of solution system and microwave radiation, have also been comparatively analyzed. With the high thermostability, thermostable glycosidases are expected to be a highly efficient and stable tool enzyme in the industrial production of glycoside compounds for hydrolysis and synthesis. |
| Key words: glycosidase clone expression glycoside synthesis site-directed mutagenesis ionic liquid microwave radiation |
